Hossy Bryan Hudson, da Costa Leitão Alvaro Augusto, Dos Santos Elisabete Pereira, Matsuda Monique, Rezende Laura Barros, Rurr Janine Simas Cardoso, Pinto Alicia Viviana, Ramos-E-Silva Marcia, de Pádula Marcelo, de Oliveira Miguel Nádia Campos
Programa de Pós-Graduação em Clínica Médica, Faculdade de Medicina - Serviço de Dermatologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Laboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
J Photochem Photobiol B. 2017 Aug;173:545-550. doi: 10.1016/j.jphotobiol.2017.06.043. Epub 2017 Jun 29.
Cosmetic preservatives are used to protect cosmetic formulations and improve its shelf-life. However, these substances may exert phototoxic effects when used under sunlight.
To assess safety, efficacy and putative phototoxic effects of a sunscreen formulation SPF 30 and its excipients.
MATERIALS/METHODS: Irradiation was performed with solar simulated light (SSL) and the sunscreen from the School of Pharmacy/UFRJ/Brazil. We used albino hairless mice in different groups (control (G1), only irradiated (G2), sunscreen plus irradiation (G3) and vehicle plus irradiation (G4) for morphological assessment and immunefluorescence detection to OKL38. In vitro analyses were with a Saccharomyces cerevisiae (SC) strain plus SSL in the presence of methylparaben, propylparaben, imidazolidinyl urea, aminomethyl propanol and their association.
G3 and G4 displayed photosensitization leading to thickening of the epidermis and increased dermal cellularity. G4 displayed strong OKL38 labeling when compared with other groups. Aminomethyl propanol, methylparaben and propylparaben are endowed with phototoxic activity against SC. Propylparaben displayed the highest phototoxic effect, followed by excipients association.
The sunscreen's vehicle is endowed with phototoxic activity. Propylparaben was the most phototoxic agent, increasing the overall phototoxicity of excipient association, pointing to a critical concern regarding vehicle associations intended to cosmetic purposes.
化妆品防腐剂用于保护化妆品配方并延长其保质期。然而，这些物质在阳光下使用时可能会产生光毒性作用。
评估一款防晒指数为30的防晒配方及其辅料的安全性、有效性和假定的光毒性作用。
材料/方法：使用太阳模拟光（SSL）对来自巴西里约热内卢联邦大学药学院的防晒霜进行照射。我们将不同组的白化无毛小鼠（对照组（G1）、仅接受照射组（G2）、涂抹防晒霜并接受照射组（G3）和涂抹赋形剂并接受照射组（G4））用于形态学评估和OKL38免疫荧光检测。体外分析是在存在对羟基苯甲酸甲酯、对羟基苯甲酸丙酯、咪唑烷基脲、氨甲基丙醇及其组合的情况下，使用酿酒酵母（SC）菌株加SSL进行的。
G3组和G4组出现光致敏现象，导致表皮增厚和真皮细胞增多。与其他组相比，G4组显示出强烈的OKL38标记。氨甲基丙醇、对羟基苯甲酸甲酯和对羟基苯甲酸丙酯对酿酒酵母具有光毒性活性。对羟基苯甲酸丙酯表现出最高的光毒性作用，其次是辅料组合。
该防晒霜的赋形剂具有光毒性活性。对羟基苯甲酸丙酯是最具光毒性的试剂，增加了辅料组合的总体光毒性，这表明对于用于化妆品目的的赋形剂组合存在关键问题。
