Kuang Jie, Li Qin-Yu, Fan Fei, Shen Ning-Jia, Zhan Yong-Jie, Tang Zhao-Hui, Yu Wen-Long
Department of Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, P.R. China.
Department II of Biliary Tract Surgery, Eastern Hepatobiliary Hospital, Shanghai 200438, P.R. China.
Oncol Lett. 2017 Jul;14(1):41-46. doi: 10.3892/ol.2017.6137. Epub 2017 May 8.
X-linked ribosomal protein S4 (RPS4X) has previously been reported to be associated with cisplatin resistance and clinical outcome in bladder and ovarian cancer. However, the value of RPS4X as a diagnostic and prognostic marker in intrahepatic cholangiocarcinoma (ICC) has not yet been investigated. The present study evaluated the expression pattern, and diagnostic and prognostic value of RPS4X in patients with ICC. Retrospective analysis was performed for a total of 201 patients with intrahepatic cholangiocarcinoma, and 8 patients with inflammation of the bile duct. Immunohistochemistry was performed using tissue microarrays to characterize the expression profile of RPS4X. Receiver operating characteristic (ROC) curves, the Kaplan-Meier estimator and Cox regression analysis were applied to evaluate the potential diagnostic and prognostic value of RPS4X in ICC. RPS4X was significantly upregulated in ICC tissues compared with the inflamed bile duct tissues. When differentiating ICC from normal controls, ROC analysis of RPS4X gave an area under the curve value of 0.9030 (sensitivity, 82.59%; specificity, 100%). RPS4X expression was significantly positively correlated with serum alkaline phosphatase levels. Survival analysis demonstrated that RPS4X expression levels were an independent prognostic factor for overall survival. Therefore, RPS4X expression levels may serve as a novel diagnostic and prognostic marker in ICC.
X连锁核糖体蛋白S4（RPS4X）此前已被报道与膀胱癌和卵巢癌中的顺铂耐药性及临床预后相关。然而，RPS4X作为肝内胆管癌（ICC）诊断和预后标志物的价值尚未得到研究。本研究评估了RPS4X在ICC患者中的表达模式及其诊断和预后价值。对总共201例肝内胆管癌患者和8例胆管炎患者进行了回顾性分析。使用组织芯片进行免疫组织化学以表征RPS4X的表达谱。应用受试者工作特征（ROC）曲线、Kaplan-Meier估计法和Cox回归分析来评估RPS4X在ICC中的潜在诊断和预后价值。与发炎的胆管组织相比，RPS4X在ICC组织中显著上调。在将ICC与正常对照区分开时，RPS4X的ROC分析得出曲线下面积值为0.9030（敏感性，82.59%；特异性，100%）。RPS4X表达与血清碱性磷酸酶水平显著正相关。生存分析表明，RPS4X表达水平是总生存的独立预后因素。因此，RPS4X表达水平可能作为ICC中的一种新型诊断和预后标志物。
