Kim Green, Kim Tae-Hyoun, Hwang Eun-Ha, Chang Kyu-Tae, Hong Jung Joo, Park Jong-Hwan
Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea.
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Chungcheongbuk 28116, Republic of Korea.
Oncol Lett. 2017 Jul;14(1):416-422. doi: 10.3892/ol.2017.6169. Epub 2017 May 12.
Human gastric adenocarcinoma (AGS) is one of the most common types of malignant tumor and the third-leading cause of tumor-associated mortality worldwide. Withaferin A (WA), a steroidal lactone derived from , exhibits antitumor activity in a variety of cancer models. However, to the best of our knowledge, the direct effect of WA on AGS cells has not previously been determined. The present study investigated the effects of WA on the proliferation and metastatic activity of AGS cells. WA exerted a dose-dependent cytotoxic effect on AGS cells. The effect was associated with cell cycle arrest at the G2/M phase and the expression of apoptotic proteins. Additionally, WA treatment resulted in a decrease in the migration and invasion ability of the AGS cells, as demonstrated using a wound healing assay and a Boyden chamber assay. These results indicate that WA directly inhibits the proliferation and metastatic activity of gastric cancer cells, and suggest that WA may be developed as a drug for the treatment of gastric cancer.
人胃腺癌（AGS）是最常见的恶性肿瘤类型之一，也是全球肿瘤相关死亡的第三大原因。从 中提取的甾体内酯 Withaferin A（WA）在多种癌症模型中均表现出抗肿瘤活性。然而，据我们所知，WA 对 AGS 细胞的直接作用此前尚未确定。本研究调查了 WA 对 AGS 细胞增殖和转移活性的影响。WA 对 AGS 细胞具有剂量依赖性细胞毒性作用。该作用与细胞周期停滞于 G2/M 期以及凋亡蛋白的表达有关。此外，如采用伤口愈合试验和 Boyden 小室试验所示，WA 处理导致 AGS 细胞的迁移和侵袭能力下降。这些结果表明，WA 直接抑制胃癌细胞的增殖和转移活性，并提示 WA 可能被开发为一种治疗胃癌的药物。
