Huang Shucheng, Rehman Mujeeb U, Qiu Gang, Luo Houqiang, Iqbal Muhammad K, Zhang Hui, Mehmood Khalid, Li Jiakui
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Laboratory of Detection and Monitoring of Highland Animal Disease, Tibet Agriculture and Animal Husbandry College, Linzhi 860000, Tibet, China.
J Vet Sci. 2018 Jan 31;19(1):107-115. doi: 10.4142/jvs.2018.19.1.107.
Tibial dyschondroplasia (TD) cases has not been reported in Tibetan chickens (TBCs), but it is commonly seen in commercial broilers characterized by lameness. The underlying mechanism remains unclear. Hypoxia-inducible factors (HIFs) are important regulators of cellular adaptation to hypoxic conditions. In this study, we investigated the role of HIF-1α, -2α, and -3α in hypoxia and thiram-induced TD and their effect on tibial growth plate development in Arbor Acres chickens (AACs) and TBCs. RNA and protein expression levels of HIF-1α, -2α, and -3α were determined by using quantitative reverse transcriptase polymerase chain reaction and western blotting analyses, respectively. Interestingly, the results showed that HIF-1α, -2α, and -3α expressions in the tibial growth plate of TBCs were upregulated by hypoxia and the change was more significant in TBCs than in AACs. However, these factors were downregulated in thiram-induced TD. To further clarify the effect of thiram on tibial growth plate in commercial broilers, AACs were observed to exhibit more pronounced changes in their growth plate that that in TBCs. Taken together, these results demonstrate that HIF-1α, -2α, and -3α may be important in tibial growth plate development and in the prevention of TD. The present study contributes novel insights on a therapeutic target for poultry TD.
藏鸡尚未报道有胫骨软骨发育不良（TD）病例，但在以跛行为特征的商品肉鸡中较为常见。其潜在机制尚不清楚。缺氧诱导因子（HIFs）是细胞适应缺氧条件的重要调节因子。在本研究中，我们调查了HIF-1α、-2α和-3α在缺氧和福美双诱导的TD中的作用及其对爱拔益加肉鸡（AACs）和藏鸡（TBCs）胫骨生长板发育的影响。分别使用定量逆转录聚合酶链反应和蛋白质印迹分析来测定HIF-1α、-2α和-3α的RNA和蛋白质表达水平。有趣的是，结果表明，缺氧可上调TBCs胫骨生长板中HIF-1α、-2α和-3α的表达，且TBCs中的变化比AACs中更显著。然而，在福美双诱导的TD中这些因子表达下调。为进一步阐明福美双对商品肉鸡胫骨生长板的影响，观察到AACs生长板的变化比TBCs更明显。综上所述，这些结果表明HIF-1α、-2α和-3α可能在胫骨生长板发育和预防TD中起重要作用。本研究为家禽TD的治疗靶点提供了新的见解。
