Gene Transfer Technology Group, Institute for Women's Health, University College London, 86-96 Chenies Mews, London WC1E 6HX, United Kingdom.
Gene Transfer Technology Group, Institute for Women's Health, University College London, 86-96 Chenies Mews, London WC1E 6HX, United Kingdom.
Methods. 2017 Aug 15;127:69-78. doi: 10.1016/j.ymeth.2017.07.001. Epub 2017 Jul 8.
Infectious diseases are one of the leading causes of death worldwide. Modelling and understanding human infection is imperative to developing treatments to reduce the global burden of infectious disease. Bioluminescence imaging is a highly sensitive, non-invasive technique based on the detection of light, produced by luciferase-catalysed reactions. In the study of infectious disease, bioluminescence imaging is a well-established technique; it can be used to detect, localize and quantify specific immune cells, pathogens or immunological processes. This enables longitudinal studies in which the spectrum of the disease process and its response to therapies can be monitored. Light producing transgenic rodents are emerging as key tools in the study of host response to infection. Here, we review the strategies for identifying biological processes in vivo, including the technology of bioluminescence imaging and illustrate how this technique is shedding light on the host-pathogen relationship.
传染病是全球主要死因之一。对人类感染进行建模和理解对于开发治疗方法以减轻全球传染病负担至关重要。生物发光成像是一种基于检测光的高度敏感、非侵入性技术，由荧光素酶催化反应产生。在传染病研究中，生物发光成像是一种成熟的技术；它可用于检测、定位和定量特定的免疫细胞、病原体或免疫过程。这使得可以进行纵向研究，监测疾病过程的范围及其对治疗的反应。发光转基因啮齿动物正在成为研究宿主对感染反应的重要工具。在这里，我们回顾了体内鉴定生物过程的策略，包括生物发光成像技术，并说明了该技术如何揭示宿主-病原体关系。
