Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
Life Sci. 2017 Sep 1;184:18-24. doi: 10.1016/j.lfs.2017.07.007. Epub 2017 Jul 8.
Taurine has been widely evaluated as a potential therapeutic agent in chronic inflammatory disorders and various infections. However, the potential role of taurine in regulating allergic inflammatory responses is currently unknown.
The present study was designed to evaluate the in vitro effects of taurine on the levels of thymic stromal lymphopoietin (TSLP) and other pro-inflammatory cytokines and activation of caspase-1 and nuclear factor (NF)-κB as well as the phosphorylations of c-Jun N-terminal kinase (JNK) and p38 in phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-triggered human mast cell line, HMC-1 cells. Furthermore, we assessed the therapeutic effects of taurine on ovalbumin (OVA)-induced allergic rhinitis (AR) animal models.
Here, the obtained results showed that taurine dose-dependently inhibited the production and mRNA expression of TSLP and pro-inflammatory cytokines in HMC-1 cells exposed to PMACI. Taurine attenuated the phosphorylation of JNK and p38 in activated HMC-1 cells. Moreover, taurine brought a significant inhibition of the activities of NF-κB and caspase-1. In an OVA-induced AR animal model, the increased levels of nose rubbing, histamine, immunoglobulin E, TSLP, and interleukin IL-1β were dramatically reduced by the administration of taurine. In summary, taurine could serve as potential novel remedy of allergic inflammatory disorders.
牛磺酸已被广泛评估为慢性炎症性疾病和各种感染的潜在治疗药物。然而，牛磺酸在调节过敏炎症反应中的潜在作用目前尚不清楚。
本研究旨在评估牛磺酸对胸腺基质淋巴细胞生成素（TSLP）和其他促炎细胞因子水平以及半胱天冬酶-1和核因子（NF）-κB的激活以及原癌基因 c-Jun N-末端激酶（JNK）和 p38 在佛波醇 12-肉豆蔻酸 13-乙酸酯和钙离子载体 A23187（PMACI）触发的人肥大细胞系 HMC-1 细胞中的磷酸化的体外影响。此外，我们评估了牛磺酸对卵清蛋白（OVA）诱导的过敏性鼻炎（AR）动物模型的治疗作用。
结果表明，牛磺酸可剂量依赖性地抑制 PMACI 处理的 HMC-1 细胞中 TSLP 和促炎细胞因子的产生和 mRNA 表达。牛磺酸减弱了活化的 HMC-1 细胞中 JNK 和 p38 的磷酸化。此外，牛磺酸对 NF-κB 和半胱天冬酶-1 的活性有显著抑制作用。在 OVA 诱导的 AR 动物模型中，牛磺酸的给药显著降低了鼻擦、组胺、免疫球蛋白 E、TSLP 和白细胞介素 IL-1β 的水平。总之，牛磺酸可以作为治疗过敏炎症性疾病的潜在新型药物。
