Wang Zhaokun, Zhang Qiongwen, Luo Linda, Sun Tiemin, Guo Xingjie
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P.R. China.
Department of Pharmaceutical Chemistry, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, Liaoning Province, P.R. China.
Chirality. 2017 Sep;29(9):558-565. doi: 10.1002/chir.22731. Epub 2017 Jul 11.
Three kinds of sulfated β-cyclodextrin (S-β-CD), including a single isomer, heptakis-6-sulfato-β-cyclodextrin (HS-β-CD), degree of substitution (DS) of 7, which was synthesized in our laboratory and another two commercialized randomly substituted mixtures, a sulfated β-cyclodextrin with DS of 7 to 11, as well as a highly sulfated-β-cyclodextrin with DS of 12 to 15, were used for the enantioresolution of 12 drugs (the β-blockers, phenethylamines, and anticholinergic agents) in capillary electrophoresis. The enantioseparation under varying concentrations of S-β-CD and background electrolyte pH were systematically investigated and compared. Based on the experimental results, the effect of the nature of S-β-CD and analyte structure on the enantioseparation is discussed.
三种硫酸化β-环糊精（S-β-CD），包括一种单一异构体，即七（6-硫酸根）-β-环糊精（HS-β-CD），其取代度（DS）为7，由我们实验室合成，另外两种是商业化的随机取代混合物，一种取代度为7至11的硫酸化β-环糊精，以及一种取代度为12至15的高硫酸化β-环糊精，用于在毛细管电泳中对12种药物（β-阻滞剂、苯乙胺类和抗胆碱能药物）进行对映体拆分。系统研究并比较了不同浓度的S-β-CD和背景电解质pH值下的对映体分离情况。基于实验结果，讨论了S-β-CD的性质和分析物结构对对映体分离的影响。
