Yao Jianfeng, Geng Lixia, Huang Rongfu, Peng Weilin, Chen Xuan, Jiang Xiaohong, Yu Miao, Li Ming, Huang Yanfang, Yang Xiaoyu
College of Preclinical Medicine, Fujian Medical University, Fuzhou, People's Republic of China
The Second Affiliated HospitalFujian Medical University, Quanzhou, People's Republic of China.
Reproduction. 2017 Sep;154(3):97-105. doi: 10.1530/REP-16-0480.
Vitrification of embryos is a routine procedure in IVF ( fertilization) laboratories. In the present study, we aimed to investigate the effect of vitrification on mouse preimplantation embryo development , and effect on the epigenetic status of imprinted gene in mouse embryos. The blastocyst formation rate for vitrified 8-cell embryos was similar to the non-vitrified 8-cell embryos, whereas the blastocyst hatching rate was lower than that of the non-vitrified group. The expression level of major-type transcript decreased significantly in vitrified blastocysts compared with non-vitrified and blastocysts. Moreover, the global DNA methylation level in 8-cell embryos and blastocysts, and the DNA methylation at CpG island 1 (CGI1) of in blastocysts were also significantly decreased after vitrification. culture condition had no adverse effect, except for on the DNA methylation in CGI1. These results suggest that vitrification may reduce the development of mouse 8-cell embryos and affect the expression and DNA methylation of imprinted gene .
胚胎玻璃化冷冻是体外受精（IVF）实验室中的常规操作。在本研究中，我们旨在探讨玻璃化冷冻对小鼠植入前胚胎发育的影响，以及对小鼠胚胎中印迹基因表观遗传状态的影响。玻璃化冷冻的8细胞胚胎的囊胚形成率与未玻璃化冷冻的8细胞胚胎相似，而囊胚孵化率低于未玻璃化冷冻组。与未玻璃化冷冻的囊胚相比，玻璃化冷冻囊胚中主要类型转录本的表达水平显著降低。此外，玻璃化冷冻后，8细胞胚胎和囊胚中的整体DNA甲基化水平以及囊胚中印记基因1（IGF2）启动子区域CpG岛1（CGI1）处的DNA甲基化也显著降低。除了对IGF2启动子区域CpG岛1处的DNA甲基化有影响外，培养条件没有不良影响。这些结果表明，玻璃化冷冻可能会降低小鼠8细胞胚胎的发育，并影响印记基因的表达和DNA甲基化。
