Friedman-Alvermann B, Müller D, Spies K
Acta Virol. 1985 Dec;29(6):482-6.
Hepatitis A virus (HAV) was isolated directly from human faeces in PLC/PRF/5 cells. In the first passage cell-bound and supernatant viruses were found by immune electron microscopy and by enzymeimmunoassay. Serial passaging of HAV in PLC/PRF/5 cells resulted in its adaptation to the cell line and in reduction of the incubation time. HAV was still detectable after 10 cell passages. Cell-bound as well as supernatant HAV were employed as antigens in anti HAV IgM-enzymeimmunoassay.
甲型肝炎病毒（HAV）直接从人粪便中分离出来，接种于PLC/PRF/5细胞。在首次传代时，通过免疫电子显微镜和酶免疫测定法发现了细胞结合病毒和上清液病毒。HAV在PLC/PRF/5细胞中连续传代导致其适应该细胞系，并缩短了孵育时间。在传代10次后仍可检测到HAV。细胞结合型和上清液型HAV均用作抗HAV IgM酶免疫测定中的抗原。
