Tesh R B, Peleg J, Samina I, Margalit J, Bodkin D K, Shope R E, Knudson D
Am J Trop Med Hyg. 1986 Mar;35(2):418-28. doi: 10.4269/ajtmh.1986.35.418.
The antigenic and biological characteristics of a new Orbivirus, designated Netivot virus, are described. This agent was originally recovered in cultures of the C6/36 clone of Aedes albopictus cells from a pool of Culex pipiens captured in Israel. Netivot virus is not pathogenic for newborn mice, nor did it initially produce detectable cytopathic effect (CPE) in Vero cells. It is closely related antigenically to Umatilla and Llano Seco viruses; these 3 agents appear to constitute a new serogroup within the genus Orbivirus. Netivot virus is also more distantly related to a number of other orbiviruses in the blue-tongue, epizootic hemorrhagic disease of deer, and Eubenangee serogroups. Netivot virus replicated to high titer and produced CPE in a variety of mosquito cell cultures, but it did not grow in 2 sand fly cell lines. Inoculation of Ae. aegypti and Ae. albopictus with Netivot virus resulted in almost 100% mortality in both species within 15 days after infection. The recovery of this and a number of other yet unidentified viral agents from field-collected mosquitoes in cultures of C6/36 cells, but not in the conventional vertebrate assay systems, suggests the existence in nature of many yet unrecognized mosquito-associated viruses. It also demonstrates the value of using new isolation methods in arbovirus studies.
本文描述了一种新的环状病毒——内蒂沃特病毒的抗原和生物学特性。该病原体最初是从以色列采集的尖音库蚊样本中，在白纹伊蚊细胞的C6/36克隆培养物中分离出来的。内蒂沃特病毒对新生小鼠无致病性，最初在非洲绿猴肾细胞中也未产生可检测到的细胞病变效应（CPE）。它在抗原性上与尤马蒂拉病毒和拉诺塞科病毒密切相关；这三种病原体似乎构成了环状病毒属内的一个新血清群。内蒂沃特病毒与蓝舌病、鹿流行性出血病和尤贝南吉血清群中的许多其他环状病毒也有较远的亲缘关系。内蒂沃特病毒在多种蚊细胞培养物中能复制到高滴度并产生CPE，但在两种白蛉细胞系中不能生长。用内蒂沃特病毒接种埃及伊蚊和白纹伊蚊，在感染后15天内两种蚊子的死亡率几乎都达到了100%。在C6/36细胞培养物中从野外采集的蚊子中分离出这种病毒和许多其他尚未鉴定的病毒病原体，但在传统的脊椎动物检测系统中未分离到，这表明自然界中存在许多尚未被认识的与蚊子相关的病毒。这也证明了在虫媒病毒研究中使用新分离方法的价值。
