镝/铽杂化高分子配合物用于动脉血栓形成的双模态成像。

Gadolinium/terbium hybrid macromolecular complexes for bimodal imaging of atherothrombosis.

机构信息

University Paris 13, Institut Galilée, University Sorbonne Paris-Cité, Laser Physics Laboratory, CNRS UMR 7538, Villetaneuse, FrancebUniversity Paris 13, Institut Galilée, University Sorbonne Paris-Cité, Laboratory for Vascular Translational Science, INSERM U1148, Villetaneuse, France.

University Paris 13, Institut Galilée, University Sorbonne Paris-Cité, Laser Physics Laboratory, CNRS UMR 7538, Villetaneuse, France.

出版信息

J Biomed Opt. 2017 Jul 1;22(7):76004. doi: 10.1117/1.JBO.22.7.076004.

DOI:10.1117/1.JBO.22.7.076004

PMID:28697231

Abstract

We developed a fluorescence imaging microscope system intended for the localization within artery slices of a gadolinium-based macromolecular biospecific magnetic resonance (MR) contrast agent used for the visualization of atherothrombosis. As the contrast agent is not initially fluorescent, we substitute some gadolinium ions for terbium ions to make them fluorescent while preserving their chemical characteristics. A long fluorescence emission time constant enables us to have a suitable signal-to-noise ratio, despite a low intensity, using pulsed illumination and time-gated imaging. Images of rat arteries show that the contrast agent is indeed localized on the specific regions of the tissues. We currently have a tool that allows us to understand and optimize the MR contrast agent.

摘要

我们开发了一种荧光显微镜成像系统，旨在对动脉切片进行定位，以检测用于可视化动脉粥样血栓形成的镧系元素基大分子生物磁共振（MR）对比剂。由于对比剂最初没有荧光，我们用铽离子替代部分镝离子，使它们在保持化学特性的同时具有荧光。长的荧光发射时间常数使我们能够在使用脉冲照明和时间门控成像时，尽管强度较低，但仍具有合适的信噪比。大鼠动脉的图像表明，对比剂确实定位于组织的特定区域。我们目前拥有一种工具，可以帮助我们了解和优化磁共振对比剂。