Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, People's Republic of China.
Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, People's Republic of China.
Bioresour Technol. 2017 Nov;243:512-519. doi: 10.1016/j.biortech.2017.06.167. Epub 2017 Jul 1.
Thermophilic microbial fuel cell (TMFC) offers many benefits, but the investigations on the diversity of exoelectrogenic bacteria are scarce. In this study, a two-chamber TMFC was constructed using ethanol as an electron donor, and the microbial dynamics were analyzed by high-throughput sequencing and 16S rRNA clone-library sequencing. The open-circuit potential of TMFC was approximately 650mV, while the maximum voltage was around 550mV. The maximum power density was 437mW/m, and the columbic efficiency in this work was 20.5±6.0%. The Firmicutes bacteria, related to the uncultured bacterium clone A55_D21_H_B_C01 with a similarity of 99%, accounted for 90.9% of all bacteria in the TMFC biofilm. This unknown bacterium has the potential to become a new thermophilic exoelectrogenic bacterium that is yet to be cultured. The development of TMFC-involved biotechnologies will be beneficial for the production of valuable chemicals and generation of energy in the future.
嗜热微生物燃料电池 (TMFC) 具有许多优势，但对其产电微生物的多样性研究还很匮乏。本研究以乙醇为电子供体构建了两室 TMFC，通过高通量测序和 16S rRNA 克隆文库测序分析了微生物动力学。TMFC 的开路电位约为 650mV，最大电压约为 550mV。最大功率密度为 437mW/m，本工作的库仑效率为 20.5±6.0%。厚壁菌门细菌占 TMFC 生物膜中所有细菌的 90.9%，与未培养菌克隆 A55_D21_H_B_C01 的相似度为 99%，该未知细菌有潜力成为一种新的尚未培养的嗜热产电菌。TMFC 相关生物技术的发展将有利于未来有价值化学品的生产和能源的产生。
