State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin, 150090, China.
School of Engineering, Northeast Agriculture University, 59 Mucai Street, Xiangfang District, Harbin, 150030, China.
Environ Sci Pollut Res Int. 2017 Oct;24(29):23226-23235. doi: 10.1007/s11356-017-9959-4. Epub 2017 Aug 22.
Microbial fuel cell (MFC) is a sustainable technology to treat cattle manure slurry (CMS) for converting chemical energy to bioelectricity. In this work, two types of allochthonous inoculum including activated sludge (AS) and domestic sewage (DS) were added into the MFC systems to enhance anode biofilm formation and electricity generation. Results indicated that MFCs (AS + CMS) obtained the maximum electricity output with voltage approaching 577 ± 7 mV (~ 196 h), followed by MFCs (DS + CMS) (520 ± 21 mV, ~ 236 h) and then MFCs with autochthonous inoculum (429 ± 62 mV, ~ 263.5 h). Though the raw cattle manure slurry (RCMS) could facilitate electricity production in MFCs, the addition of allochthonous inoculum (AS/DS) significantly reduced the startup time and enhanced the output voltage. Moreover, the maximum power (1.259 ± 0.015 W/m) and the highest COD removal (84.72 ± 0.48%) were obtained in MFCs (AS + CMS). With regard to microbial community, Illumina HiSeq of the 16S rRNA gene was employed in this work and the exoelectrogens (Geobacter and Shewanella) were identified as the dominant members on all anode biofilms in MFCs. For anode microbial diversity, the MFCs (AS + CMS) outperformed MFCs (DS + CMS) and MFCs (RCMS), allowing the occurrence of the fermentative (e.g., Bacteroides) and nitrogen fixation bacteria (e.g., Azoarcus and Sterolibacterium) which enabled the efficient degradation of the slurry. This study provided a feasible strategy to analyze the anode biofilm formation by adding allochthonous inoculum and some implications for quick startup of MFC reactors for CMS treatment.
微生物燃料电池（MFC）是一种可持续的技术，可用于处理牛粪浆（CMS），将化学能转化为生物电能。在这项工作中，加入了两种异源接种物，包括活性污泥（AS）和生活污水（DS），以增强阳极生物膜的形成和发电。结果表明，MFC（AS + CMS）获得了最大的电量输出，电压接近 577±7 mV（196 h），其次是 MFC（DS + CMS）（520±21 mV，236 h），然后是使用同源接种物的 MFC（429±62 mV，~263.5 h）。虽然原始牛粪浆（RCMS）可以促进 MFC 中的发电，但异源接种物（AS/DS）的添加显著缩短了启动时间并提高了输出电压。此外，在 MFC（AS + CMS）中获得了最大的功率（1.259±0.015 W/m）和最高的 COD 去除率（84.72±0.48%）。关于微生物群落，本工作采用 Illumina HiSeq 16S rRNA 基因，并鉴定出在 MFC 中所有阳极生物膜上的优势微生物是好氧微生物（如 Geobacter 和 Shewanella）。就阳极微生物多样性而言，MFC（AS + CMS）优于 MFC（DS + CMS）和 MFC（RCMS），允许发酵菌（如 Bacteroides）和固氮菌（如 Azoarcus 和 Sterolibacterium）的发生，从而实现了浆体的有效降解。本研究提供了一种通过添加异源接种物分析阳极生物膜形成的可行策略，并对 CMS 处理的 MFC 反应器的快速启动具有一定的启示意义。
