Balalakshmi Chinnasamy, Gopinath Kasi, Govindarajan Marimuthu, Lokesh Ravi, Arumugam Ayyakannu, Alharbi Naiyf S, Kadaikunnan Shine, Khaled Jamal M, Benelli Giovanni
Department of Nanoscience and Technology, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
Department of Botany, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
J Photochem Photobiol B. 2017 Aug;173:598-605. doi: 10.1016/j.jphotobiol.2017.06.040. Epub 2017 Jun 29.
The impact of green-fabricated gold nanoparticles on plant cells and non-target aquatic species is scarcely studied. In this research, we reported an environment friendly technique for the synthesis of gold nanoparticles (Au NPs) using the Sphaeranthus indicus leaf extract. The formation of the metal NPs was characterized by UV-Visible and FT-IR spectroscopy, XRD, SEM and TEM analyses. The UV-Visible spectra of Au NPs showed a surface plasmon resonance peak at 531nm. FT-IR analysis indicated functional bio-molecules associated with Au NPs formation. The crystalline nature of Au nanoparticles was confirmed by their XRD diffraction pattern. TEM revealed the spherical shape with a mean particle size of 25nm. Au NPs was tested at 0, 1, 3, 5, 7 and 10% doses in mitotic cell division assays, pollen germination experiments, and in vivo toxicity trials against the aquatic crustacean Artemia nauplii. Au NPs did not show any toxic effects on plant cells and aquatic invertebrates. Notably, Au NPs promoted mitotic cell division in Allium cepa root tip cells and germination of Gloriosa superba pollen grains. Au NPs showed no mortality on A. nauplii, all the tested animals showed 100% survivability. Therefore, these Au NPs have potential applications in the development of pollen germination media and plant tissue culture.
绿色制备的金纳米颗粒对植物细胞和非靶标水生物种的影响鲜有研究。在本研究中，我们报道了一种利用印度圆叶薄荷叶提取物合成金纳米颗粒（Au NPs）的环境友好技术。通过紫外可见光谱和傅里叶变换红外光谱、X射线衍射、扫描电子显微镜和透射电子显微镜分析对金属纳米颗粒的形成进行了表征。Au NPs的紫外可见光谱在531nm处显示出表面等离子体共振峰。傅里叶变换红外分析表明了与Au NPs形成相关的功能性生物分子。通过XRD衍射图谱证实了金纳米颗粒的晶体性质。透射电子显微镜显示其呈球形，平均粒径为25nm。在有丝分裂细胞分裂试验、花粉萌发实验以及针对水生甲壳动物卤虫无节幼体的体内毒性试验中，对Au NPs进行了0%、1%、3%、5%、7%和10%剂量的测试。Au NPs对植物细胞和水生无脊椎动物未显示出任何毒性作用。值得注意的是，Au NPs促进了洋葱根尖细胞的有丝分裂细胞分裂以及大花嘉兰花粉粒的萌发。Au NPs对卤虫无节幼体未显示出致死性，所有受试动物的存活率均为100%。因此，这些Au NPs在花粉萌发培养基和植物组织培养的开发中具有潜在应用价值。
