Chen Yan-Zhao, Liu Zhi-Ping, Zhou Ke-Ying, Li Bo
Department of Pediatrics, Shenzhen People's Hospital, Second Clinical Medical College of Jinan University, Shenzhen, Guangdong 518020, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2017 Jul;19(7):776-780. doi: 10.7499/j.issn.1008-8830.2017.07.009.
To investigate the value of serum miR-17-92 cluster in the diagnosis of retinoblastoma (RB).
Serum samples were collected from 20 children with RB and 20 healthy controls. Quantitative real-time PCR was used to measure the expression of miR-17-92 cluster. The expression of miR-17-92 cluster was compared between children with different stages of RB and the changes in the expression of miR-17-92 cluster after multimodality therapy were analyzed. The receiver operating characteristic (ROC) curve was used to investigate the value of serum miR-17-92 cluster in the diagnosis of RB.
Compared with the healthy controls, the children with RB had significantly higher relative expression of miR-17-3P, miR-17-5P, miR-18a, and miR-20a in serum (P<0.05), and miR-18a showed the greatest increase. There were no significant differences in the relative expression of miR-19a, miR-19b-1, and miR-92a-1 between children with RB and healthy controls (P>0.05). There were no significant differences in the expression of miR-17-5P, miR-17-3P, miR-18a, and miR-20a between the children with early-to-moderate stage of RB and those with advanced stage of RB (P>0.05), but there were significant reductions after multimodality therapy (P<0.05). In the diagnosis of RB, the areas under the ROC curve (AUCs) for serum miR-17-3P, miR-17-5P, miR-18a, and miR-20a were 0.770, 0.755, 0.828, and 0.665 respectively, and miR-18a had the largest AUC, with a sensitivity of 90% and a specificity of 65%.
miR-17-3P, miR-17-5P, miR-18a, and miR-20a are highly expressed in the serum of children with RB, and miR-18a may be used as a new marker for the diagnosis of RB.
探讨血清miR-17-92簇在视网膜母细胞瘤（RB）诊断中的价值。
收集20例RB患儿和20例健康对照者的血清样本。采用定量实时荧光定量PCR检测miR-17-92簇的表达。比较不同分期RB患儿miR-17-92簇的表达情况，并分析多模态治疗后miR-17-92簇表达的变化。采用受试者工作特征（ROC）曲线研究血清miR-17-92簇在RB诊断中的价值。
与健康对照者相比，RB患儿血清中miR-17-3P、miR-17-5P、miR-18a和miR-20a的相对表达显著升高（P<0.05），其中miR-18a升高最为明显。RB患儿与健康对照者之间miR-19a、miR-19b-1和miR-92a-1的相对表达无显著差异（P>0.05）。早中期RB患儿与晚期RB患儿之间miR-17-5P、miR-17-3P、miR-18a和miR-20a的表达无显著差异（P>0.05），但多模态治疗后显著降低（P<0.05）。在RB诊断中，血清miR-17-3P、miR-17-5P、miR-18a和miR-20a的ROC曲线下面积（AUC）分别为0.770、0.755、0.828和0.665，其中miR-18a的AUC最大，灵敏度为90%，特异度为65%。
miR-17-3P、miR-17-5P、miR-18a和miR-20a在RB患儿血清中高表达，miR-18a可作为RB诊断的新标志物。
