Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Cell Biol Int. 2012 Nov 1;36(11):1005-12. doi: 10.1042/CBI20110089.
The miR-17-92 cluster is composed of seven miRNAs (microRNAs; miR-17-5p, miR-17-3p, miR-18a, miR-19a, miR-20a, miR-19b-1 and miR-92a-1). Previous studies have indicated that this cluster is involved in cell proliferation and their overexpression has been seen in several types of cancer. We have assessed the overexpression effects of miR-17-92 on the expression of several genes associated with cell-cycle regulation. The human miR-17-92 gene was cloned into a transposone-based vector, piggyBac and transfected into HEK-293T [HEK-293 cells (human embryonic kidney cells) expressing the large T-antigen of SV40 (simian virus 40)] cell line. Gene expression analysis indicated that up-regulation of this cluster causes significant changes in the expression of several cell-cycle related genes, including CDK2 (cyclin-dependent kinase 2), cyclin-D2, c-Myc and CREB (cAMP-response-element-binding protein). Other methods of transcripts assessment confirmed miR-17-92 overexpression enhances cell proliferation.
miR-17-92 簇由七个 miRNAs（microRNAs；miR-17-5p、miR-17-3p、miR-18a、miR-19a、miR-20a、miR-19b-1 和 miR-92a-1）组成。先前的研究表明，该簇参与细胞增殖，并且在几种类型的癌症中观察到其过表达。我们评估了 miR-17-92 对与细胞周期调控相关的几个基因表达的过表达效应。将人 miR-17-92 基因克隆到基于转座子的载体 piggyBac 中，并转染到 HEK-293T[HEK-293 细胞（人胚肾细胞）表达 SV40（猿猴病毒 40）的大 T 抗原]细胞系中。基因表达分析表明，该簇的上调导致与细胞周期相关的几个基因的表达发生显著变化，包括 CDK2（细胞周期蛋白依赖性激酶 2）、cyclin-D2、c-Myc 和 CREB（cAMP 反应元件结合蛋白）。其他转录物评估方法证实 miR-17-92 过表达可增强细胞增殖。
