Department of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.
Department of Thoracic and Cardiovascular Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China; Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China; Shanghai Pediatric Congenital Heart Disease Institute, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai, People's Republic of China.
J Thorac Cardiovasc Surg. 2017 Nov;154(5):1734-1739. doi: 10.1016/j.jtcvs.2017.06.016. Epub 2017 Jun 13.
To construct a neonatal rat model of increased right ventricular (RV) afterload for studying the pathophysiological remodeling of the right ventricle in patients with congenital heart disease with increased RV afterload.
Surgery was performed within 6 hours after birth. Horizontal thoracotomy was performed by dissecting the intercostal muscles and splitting the sternum. The PA was then banded with 11-0 nylon thread. At postnatal day 7 (P7), constriction of PA was confirmed by echocardiography. The RV systolic and diastolic pressures were measured by cardiac catheterization. The RV end-systolic volume, end-diastolic volume, end-diastolic diameter, and free wall thickness were assessed by magnetic resonance imaging. The histological changes in sham-operated and PA-banding (PAB) hearts were evaluated by hematoxylin and eosin staining.
Increased RV afterload was established by constriction of the PA in neonatal rats within 6 hours after birth. The survival rate was 75% at P7. Relative to the sham group, the peak pressure gradient across the PA constriction and RV systolic and diastolic pressures, end-systolic volume, end-diastolic volume, end-diastolic diameter, and free wall thickness were significantly increased in the PAB group at P7 (P < .01). Consistently, histological examination showed that the RV free wall was significantly hypertrophic in the PAB group.
We successfully established a neonatal RV afterload increase model through PAB within 6 hours after birth, which can be used to study the pathophysiological changes in congenital heart diseases with increased RV afterload.
构建右心室（RV）后负荷增加的新生大鼠模型，以研究 RV 后负荷增加的先天性心脏病患者右心室病理生理重构。
出生后 6 小时内进行手术。通过解剖肋间肌和劈开胸骨进行横开胸术。然后用 11-0 尼龙线环扎 PA。在出生后第 7 天（P7），通过超声心动图确认 PA 缩窄。通过心导管测量 RV 收缩压和舒张压。通过磁共振成像评估 RV 收缩末期容积、舒张末期容积、舒张末期直径和游离壁厚度。通过苏木精-伊红染色评估假手术和 PA 缩窄（PAB）心脏的组织学变化。
在出生后 6 小时内通过缩窄 PA 成功建立了新生大鼠 RV 后负荷增加模型。P7 时的存活率为 75%。与假手术组相比，PAB 组在 P7 时的 PA 缩窄跨壁压力梯度、RV 收缩压和舒张压、收缩末期容积、舒张末期容积、舒张末期直径和游离壁厚度显著增加（P < 0.01）。一致地，组织学检查显示 PAB 组 RV 游离壁明显肥厚。
我们通过出生后 6 小时内的 PA 缩窄成功建立了一种 RV 后负荷增加的新生大鼠模型，可用于研究 RV 后负荷增加的先天性心脏病的病理生理变化。
