University of Melbourne, Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville, Victoria, 3052, Australia; Pregnancy Research Centre, Department of Maternal-Fetal Medicine, Royal Women's Hospital, Parkville, Victoria, 3052, Australia; Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Mail Code 1515, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.
University of Melbourne, Department of Obstetrics and Gynaecology, Royal Women's Hospital, Parkville, Victoria, 3052, Australia; Pregnancy Research Centre, Department of Maternal-Fetal Medicine, Royal Women's Hospital, Parkville, Victoria, 3052, Australia.
Placenta. 2017 Nov;59:131-138. doi: 10.1016/j.placenta.2017.06.341. Epub 2017 Jun 27.
Chorionic mesenchymal stem/stromal cells (CMSC) can be isolated from the placenta in large numbers. Although their functions are yet to be fully elucidated, they have a role in tissue development and repair. To fulfil such a role, CMSC must be able to migrate to the microenvironment of the injury site. This process is not fully understood and the aim of this study therefore, was to examine in vitro CMSC migration in response to tissue inflammation and hypoxic conditioning.
CMSC were derived from the chorionic villi. A trans-endothelium migration (TEM) assay was used to study CMSC migration through an activated endothelial cell monolayer using the HMEC-1 cell line. A cytokine array was used to identify and compare the cytokine production profile of activated versus non-activated HMEC-1.
There were significant changes in cytokine production by HMEC-1 cells following lipopolysaccharide (LPS) treatment and hypoxic conditioning. Despite this, results from the TEM assay showed no significant change in the average number of CMSC that migrated through the LPS activated HMEC-1 layer compared to the untreated control. Furthermore, there was no significant change in the average number of CMSC that migrated through the HMEC-1 monolayer when exposed to hypoxic (1% O), normoxic (8% O) or hyperoxic (21% O) conditions.
These data suggest that cell functions such as transendothelial migration can vary between MSC derived from different tissues in response to the same biological cues.
绒毛膜间充质干细胞（CMSC）可从胎盘大量分离。尽管其功能尚未完全阐明，但它们在组织发育和修复中起作用。为了发挥这样的作用，CMSC 必须能够迁移到损伤部位的微环境中。这个过程尚未完全了解，因此，本研究的目的是研究 CMSC 在组织炎症和缺氧条件下的体外迁移。
CMSC 来源于绒毛膜绒毛。使用 HMEC-1 细胞系，通过激活的内皮细胞单层，通过跨内皮细胞迁移（TEM）测定来研究 CMSC 迁移。使用细胞因子阵列来鉴定和比较激活的与非激活的 HMEC-1 之间的细胞因子产生谱。
HMEC-1 细胞经脂多糖（LPS）处理和缺氧条件后，细胞因子的产生发生了显著变化。尽管如此，TEM 测定的结果显示，与未处理的对照相比，通过 LPS 激活的 HMEC-1 层迁移的 CMSC 的平均数量没有显著变化。此外，当暴露于低氧（1% O）、常氧（8% O）或高氧（21% O）条件时，通过 HMEC-1 单层迁移的 CMSC 的平均数量没有显著变化。
这些数据表明，源自不同组织的 MSC 对相同的生物学信号的跨内皮迁移等细胞功能可能不同。
