大鼠和小鼠腹腔器官活体显微镜成像的手术准备。
Surgical preparation of rats and mice for intravital microscopic imaging of abdominal organs.
机构信息
Indiana University School of Medicine, Department of Medicine, Division of Nephrology, 950 W. Walnut St, R2-234, Indianapolis, IN 46202, United States.
出版信息
Methods. 2017 Sep 1;128:129-138. doi: 10.1016/j.ymeth.2017.07.003. Epub 2017 Jul 8.
Abstract
Intravital microscopy is a powerful research tool that can provide insight into cellular and subcellular events that take place in organs in the body. However, meaningful results can only be obtained from animals whose physiology is preserved during the process of microscopy. Here I discuss the importance of preserving the overall state of health of the animal, methods of anesthesia, surgical techniques for intravital microscopy of various abdominal organs, methods to maintain and monitor the physiology of the animal during microscopy and associated peri- and post-operative recovery considerations.
摘要
活体显微镜检查是一种强大的研究工具，可以深入了解体内器官中发生的细胞和亚细胞事件。然而，只有在显微镜检查过程中保持动物生理机能的情况下，才能获得有意义的结果。在这里，我讨论了保持动物整体健康状态的重要性、麻醉方法、各种腹部器官活体显微镜检查的手术技术、在显微镜检查期间维持和监测动物生理机能的方法以及相关的围手术期和术后恢复注意事项。
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