脊髓内T细胞的活体成像

Intravital Imaging of T Cells Within the Spinal Cord.

作者信息

Kawakami Naoto

机构信息

Institute of Clinical Neuroimmunology, University Hospital and Biomedical Center, Ludwig-Maximilians University Munich, Munich, Germany.

Max-Planck Institute of Neurobiology, Martinsried, Germany.

出版信息

Methods Mol Biol. 2018;1763:119-127. doi: 10.1007/978-1-4939-7762-8_11.

DOI:10.1007/978-1-4939-7762-8_11

PMID:29476493

Abstract

Intravital imaging is a powerful tool for analyzing cellular functions in living animals. In particular, after the two-photon microscopy technique was introduced, a number of studies have visualized important processes. Here, we describe the methods for performing intravital imaging of the central nervous system. This method can be used for imaging not only lymphocytes but also blood vessels for ischemia studies, as well as glia cell activities.

摘要

活体成像技术是分析活体动物细胞功能的有力工具。特别是在引入双光子显微镜技术之后，许多研究已经可视化了重要的过程。在这里，我们描述了对中枢神经系统进行活体成像的方法。该方法不仅可用于对淋巴细胞成像，还可用于对缺血研究中的血管以及神经胶质细胞活动进行成像。