Zhao Xiao-Dan, Liu Hao, Li Tao, Gong Quan, Zhang Wen-Li
Department of Orthopedics, West China Hospital of Sichuan University, Chengdu, Sichuan, China (mainland).
Med Sci Monit. 2017 Jul 12;23:3367-3372. doi: 10.12659/msm.902477.
BACKGROUND To study the protective effects and explore the mechanism of epigallocatechingallate (EGCG) on the hip fracture-induced acute lung injury. MATERIAL AND METHODS Thirty male Sprague-Dawley (SD) rats were randomly divided into the control group, hip fracture group, and hip fracture + EGCG (10 mg/Kg) group. After 24 h, blood samples, bronchoalveolar lavage fluid (BALF), and lung tissue were collected. Serum mitochondrial DNA (mtDNA) was measured by RT-PCR and BALF was used to perform cytological analysis and enzyme-linked immunosorbent assay (ELISA) assay. Lung tissue was used to evaluate the injury level. RESULTS EGCG significantly reduced the hip fracture-induced high level of serum mtDNA (p<0.05). HE staining showed protective effects of EGCG. Lower lung injury score and wet/dry ratio were identified in the hip fracture + EGCG group than in the hip fracture group (p<0.05). We found significantly lower levels of infiltration of inflammatory cells and production of inflammatory cytokines in the BALF of the hip fracture + EGCG group than in the hip fracture group (p<0.05). CONCLUSIONS Our study found that EGCG had protective effects on hip fracture-induced acute lung injury and suggests that EGCG exerts its protective effects through limiting the release of mtDNA. Our results provide a novel pharmacological agent to attenuate hip fracture-induced acute lung injury, as well as a potential theory to better explain the anti-inflammatory property of EGCG.
背景 研究表没食子儿茶素没食子酸酯（EGCG）对髋部骨折所致急性肺损伤的保护作用并探讨其机制。材料与方法 将30只雄性Sprague-Dawley（SD）大鼠随机分为对照组、髋部骨折组和髋部骨折+EGCG（10mg/Kg）组。24小时后，采集血样、支气管肺泡灌洗液（BALF）和肺组织。通过RT-PCR检测血清线粒体DNA（mtDNA），并使用BALF进行细胞学分析和酶联免疫吸附测定（ELISA）。肺组织用于评估损伤程度。结果 EGCG显著降低了髋部骨折所致的血清mtDNA高水平（p<0.05）。HE染色显示EGCG具有保护作用。与髋部骨折组相比，髋部骨折+EGCG组的肺损伤评分和湿/干比更低（p<0.05）。我们发现，与髋部骨折组相比，髋部骨折+EGCG组BALF中的炎症细胞浸润水平和炎症细胞因子产生水平显著更低（p<0.05）。结论 我们的研究发现EGCG对髋部骨折所致急性肺损伤具有保护作用，并表明EGCG通过限制mtDNA的释放发挥其保护作用。我们的结果提供了一种减轻髋部骨折所致急性肺损伤的新型药物，以及一种更好地解释EGCG抗炎特性的潜在理论。
