Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, P.R. China.
Department of Animal Sciences, Purdue University, West Lafayette, IN, 47907-2054, USA.
Sci Rep. 2017 Jul 11;7(1):5052. doi: 10.1038/s41598-017-04336-x.
Estrus expression by gilts and sows is hereditable and important for heat detection. To better understand the molecular biological mechanisms of estrus expression in gilts, the mRNA expression profiles of follicular tissue from Large White gilts in diestrus (LD, n = 3) and estrus (LE, n = 3), and Chinese indigenous Mi gilts in diestrus (MD, n = 2) and estrus (ME, n = 3) were investigated using RNA sequencing. We detected 122,804-335,295 SNPs, 6,140-14,947 InDel and 12 types of AS events (39.57% TSS, 34.90% TTS) in 11 samples. A total of 2,838 differentially expressed genes (DEGs) were found in LD vs MD, LE vs ME, LE vs LD, or ME vs MD comparisons. Two DEGs (ACP5 and PIGS) were observed in all comparisons. Two new genes (ENSSSCG00000028235 and ENSSSCG00000021903) were exclusively expressed in Mi and Large White gilts, respectively. Bioinformatics analyses indicate that these DEGs are involved in single-organism process, catalytic activity, cell adhesion and enriched in ECM-receptor interaction, olfactory transduction, ovarian steroidogenesis, steroid biosynthesis and CAMs signaling pathways. These results of RNA-Seq have provided important information for screening the key functional genes or molecular markers of estrus expression in gilts.
发情表现可遗传，对于发情检测十分重要。为了更好地理解大白母猪和梅山母猪发情的分子生物学机制，本研究利用 RNA 测序技术，研究了长白母猪发情期（LE）和发情前期（LD）（n=3）及梅山母猪发情期（ME）和发情前期（MD）（n=3）的卵泡组织的 mRNA 表达谱。在 11 个样本中，共检测到 122804-335295 个 SNP、6140-14947 个 InDel 和 12 种 AS 事件（39.57% TSS、34.90% TTS）。在 LD 与 MD、LE 与 ME、LE 与 LD 或 ME 与 MD 的比较中，共发现 2838 个差异表达基因（DEGs）。在所有比较中，有两个 DEG（ACP5 和 PIGS）被观察到。分别在梅山猪和长白猪中发现了两个新基因（ENSSSCG00000028235 和 ENSSSCG00000021903）。生物信息学分析表明，这些 DEGs 参与了单个生物体过程、催化活性、细胞黏附和细胞外基质受体相互作用、嗅觉转导、卵巢类固醇生成、类固醇生物合成和 CAMs 信号通路的富集。这些 RNA-Seq 的结果为筛选发情母猪关键功能基因或分子标记提供了重要信息。
