Chu Q, Liang T, Zhou B
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Reprod Domest Anim. 2017 Aug;52(4):696-700. doi: 10.1111/rda.12964. Epub 2017 Mar 30.
Oestrous signs affect timely mating and reproductive efficiency in swine breeding herds. To study the genetic difference of oestrous signs between Chinese and European pigs, 100 Landrace-Large White (LLW) cross gilts and 50 Chinese Mi gilts were assessed for oestrous signs and the concentrations of serum estradiol-17β and progesterone were determined. The genotype of 39 single nucleotide polymorphisms (SNPs) in 11 oestrogen metabolism and function-related genes was determined by Sequenom iPLEX platform. Compared with LLW gilts, Mi gilts had longer time of standing reflex (p < .001), higher scores of vulva reddening (p = .001) and greater serum estradiol-17β concentration (p < .01). Gilts with greater serum estradiol-17β concentrations also had greater (p < .05) scores for oestrous signs. Genetic polymorphisms of nine genes in oestrogen metabolism pathways had significant differences (p < .05) between LLW and Mi gilts. There were three and six haploblocks of SNPs in LLW and Mi, respectively. Compared with LLW, the distribution of haplotypes was more centralized in Mi pigs. Genetic polymorphisms of oestrogen metabolism-related genes have considerable differences between Chinese Mi and European LLW pigs. Because of the important roles of oestrogen during the oestrus, some genes of oestrogen metabolism pathway could be considered as candidate genes for oestrous signs.
发情迹象影响猪育种群体的适时配种和繁殖效率。为研究中国猪和欧洲猪之间发情迹象的遗传差异，对100头长白-大白（LLW）杂交后备母猪和50头中国米猪后备母猪的发情迹象进行了评估，并测定了血清雌二醇-17β和孕酮的浓度。通过Sequenom iPLEX平台测定了11个雌激素代谢和功能相关基因中39个单核苷酸多态性（SNP）的基因型。与LLW后备母猪相比，米猪后备母猪的静立反射时间更长（p <.001），外阴红肿评分更高（p =.001），血清雌二醇-17β浓度更高（p <.01）。血清雌二醇-17β浓度较高的后备母猪发情迹象评分也更高（p <.05）。雌激素代谢途径中9个基因的遗传多态性在LLW和米猪后备母猪之间存在显著差异（p <.05）。LLW和米猪分别有3个和6个SNP单倍型模块。与LLW相比，米猪的单倍型分布更集中。中国米猪和欧洲LLW猪之间雌激素代谢相关基因的遗传多态性存在相当大的差异。由于雌激素在发情期的重要作用，雌激素代谢途径中的一些基因可被视为发情迹象的候选基因。
