Rosenberg J, Hurwitz S, Bar A
Comp Biochem Physiol A Comp Physiol. 1986;83(2):277-81. doi: 10.1016/0300-9629(86)90574-8.
The possible involvement of plasma calcium and 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] in the regulation of the concentration of kidney calcium-binding protein (CaBP) was investigated. Chicks were fed diets varying in Ca2+ and P, with or without vitamin D. CaBP and 1,25(OH)2D3 were determined by competitive binding assays. A significant correlation between plasma and kidney 1,25(OH)2D3 was found, the linear regression equation of best-fit was plasma 1,25(OH)2D3 = 0.14 + 1.56 kidney 1,25(OH)2D3. In the vitamin D-fed chicks, kidney CaBP varied independently of the circulating or organ level of 1,25(OH)2D3 (P greater than 0.05), but was lower in the vitamin D-deficient than in the vitamin D-fed birds. A significant correlation was observed between kidney CaBP and plasma calcium (Cap). The regression equations were CaBP = Cap/(85.57-4.00 Cap) (R = 0.845) and CaBP = 0.0558 + 0.0404 Cap (R = 0.749), for vitamin D-treated and vitamin D-deficient chicks, respectively. The results suggest that the concentration of kidney CaBP is modulated by plasma calcium, but one or more of the vitamin D metabolites may be required for its synthesis.
研究了血浆钙和1,25 - 二羟基维生素D3[1,25(OH)2D3]在调节肾钙结合蛋白（CaBP）浓度中的可能作用。给雏鸡喂食钙和磷含量不同的日粮，添加或不添加维生素D。通过竞争性结合试验测定CaBP和1,25(OH)2D3。发现血浆和肾1,25(OH)2D3之间存在显著相关性，最佳拟合的线性回归方程为血浆1,25(OH)2D3 = 0.14 + 1.56×肾1,25(OH)2D3。在喂食维生素D 的雏鸡中，肾CaBP的变化独立于1,25(OH)2D3的循环或器官水平（P大于0.05），但维生素D缺乏的雏鸡肾CaBP低于喂食维生素D的雏鸡。观察到肾CaBP与血浆钙（Cap）之间存在显著相关性。对于经维生素D处理和维生素D缺乏的雏鸡，回归方程分别为CaBP = Cap/(85.57 - 4.00 Cap)（R = 0.845）和CaBP = 0.0558 + 0.0404 Cap（R = 0.749）。结果表明，肾CaBP的浓度受血浆钙调节，但其合成可能需要一种或多种维生素D代谢产物。
