Daneo L S, Corvetti G, Panattoni G L
Department of Human Anatomy and Physiology, University of Turin, Italy.
Cell Tissue Res. 1995 Jan;279(1):209-13. doi: 10.1007/BF00300705.
The ontogeny of two calcium-binding proteins (calbindin-D28k and calretinin) was studied by immunohistochemical techniques in developing chick kidney. This study showed the presence of calbindin on the 5th incubation day and calretinin on the 7th incubation day in mesonephric distal and connecting tubules, and in the medial wall of the Wolffian duct. At later stages, immunostaining for these two proteins, in particular for calretinin, was also demonstrated in some metanephric proximal tubules. Glomeruli and Bowman's capsules were negative both in the mesonephros and metanephros. The presence of calretinin in the developing kidney has thus been demonstrated for the first time. The early expression of calbindin and calretinin in mesonephric distal tubules suggests their role in regulating the final excretion of calcium. The different patterns of immunoreactivity of the walls of the Wolffian duct can be correlated with their different histogenetic and histological features.
通过免疫组织化学技术研究了发育中的鸡肾中两种钙结合蛋白（钙结合蛋白-D28k和钙视网膜蛋白）的个体发生。该研究表明，在中肾远端和连接小管以及沃尔夫管内壁中，钙结合蛋白在孵化第5天出现，钙视网膜蛋白在孵化第7天出现。在后期阶段，这两种蛋白质，特别是钙视网膜蛋白，在一些后肾近端小管中也显示出免疫染色。肾小球和鲍曼囊在中肾和后肾中均为阴性。因此，首次证明了钙视网膜蛋白在发育中的肾脏中的存在。钙结合蛋白和钙视网膜蛋白在中肾远端小管中的早期表达表明它们在调节钙的最终排泄中起作用。沃尔夫管管壁不同的免疫反应模式与其不同的组织发生和组织学特征相关。
