豆蚀叶野螟（Anticarsia gemmatalis Hübner）中细胞色素P-450依赖性单加氧酶活性

Cytochrome P-450-dependent monooxygenase activity in the velvetbean caterpillar, Anticarsia gemmatalis Hübner.

作者信息

Christian M F, Yu S J

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1986;83(1):23-7. doi: 10.1016/0742-8413(86)90007-1.

Abstract

The activity of microsomal aldrin epoxidase was observed in various tissues of the last instar larva of the velvetbean caterpillar (Anticarsia gemmatalis Hübner) with the midgut being the most active. Optimum conditions for assaying the enzyme were established. Allelochemicals (monoterpenes, indoles and flavones) and host plants (cowpeas, cotton, peanuts, soybeans and hairy indigo) were found to induce the monooxygenase. Piperonyl butoxide decreased and increased the toxicity of methomyl and methyl parathion, respectively, suggesting that the monooxygenase system of velvetbean caterpillars plays a major role in the detoxification of xenobiotics.

摘要

在豆蚀叶野螟（Anticarsia gemmatalis Hübner）末龄幼虫的各种组织中观察到微粒体艾氏剂环氧化酶的活性，其中中肠活性最高。确定了测定该酶的最佳条件。发现化感物质（单萜、吲哚和黄酮）和寄主植物（豇豆、棉花、花生、大豆和毛木蓝）可诱导单加氧酶。胡椒基丁醚分别降低和增加了灭多威和甲基对硫磷的毒性，这表明豆蚀叶野螟的单加氧酶系统在异生物质解毒中起主要作用。