Hilfer S R, Schneck S L, Brown J W
Exp Lung Res. 1986;10(2):115-36. doi: 10.3109/01902148609061488.
Differentiation of the respiratory region of fetal mouse lungs was investigated in serum-free medium supplemented with growth factors and hormones. Terminal buds from the margins of a lobe were removed from 16-day fetuses and organ cultures prepared either in submersion culture or at the air-medium interface. It was found that glycyl-L-histidyl-L-lysine, transferrin, and somatostatin were sufficient to promote branching in the absence of serum. However, type II pneumocytes containing lamellar bodies formed only in the presence of thyroxine or dexamethasone. At concentrations of these hormones slightly above the physiological range most of the cells became cuboidal and contained lamellar bodies; at lower concentrations regions of flattened cells appeared. In submersion culture a large, central cavity surrounded by saccules was formed rather than a branched tree. Thus, the pattern of differentiation is significantly influenced by culture conditions.
在添加了生长因子和激素的无血清培养基中，对胎鼠肺呼吸区的分化进行了研究。从16天龄胎儿肺叶边缘取下终末芽，制备器官培养物，分别进行浸没培养或气-液界面培养。结果发现，在无血清条件下，甘氨酰-L-组氨酰-L-赖氨酸、转铁蛋白和生长抑素足以促进分支。然而，仅在甲状腺素或地塞米松存在的情况下才会形成含有板层小体的II型肺上皮细胞。当这些激素的浓度略高于生理范围时，大多数细胞变成立方形并含有板层小体；在较低浓度时，则出现扁平细胞区域。在浸没培养中，形成的是一个由囊泡围绕的大的中央腔，而不是分支状结构。因此，分化模式受到培养条件的显著影响。
