Togami M, Blazka D, Hayashi J
Japan Synthetic Rubber Co., Ltd., Kanagawa, Japan.
In Vitro Cell Dev Biol. 1988 Jul;24(7):699-704. doi: 10.1007/BF02623608.
A serum-free, hormone and attachment factor supplemented culture for rat H4 hepatoma cells was established. In the defined medium (Dulbecco's Modified Eagle's + Ham's F12 + insulin, transferrin, fibronectin liver cell growth factor, and sodium selenite), H4 cells grew equally well as in 10% fetal bovine serum supplemented medium. H4 cells in either defined or serum-containing culture conditions produce transferrin but not albumin or alpha-fetoprotein. In this paper we have studied the effect of various hormones and pressor peptides on the production of angiotensinogen by H4 cells cultured in defined conditions. Only glucocorticoid hormone had a significant effect on the production of angiotensinogen, whereas other hormones previously reported to exert their effect on angiotensinogen production had little or no effect.
建立了一种用于大鼠H4肝癌细胞的无血清、添加激素和附着因子的培养体系。在限定培养基（杜氏改良伊格尔培养基+哈姆氏F12培养基+胰岛素、转铁蛋白、纤连蛋白、肝细胞生长因子和亚硒酸钠）中，H4细胞的生长情况与在添加10%胎牛血清的培养基中一样良好。在限定培养基或含血清的培养条件下，H4细胞均产生转铁蛋白，但不产生白蛋白或甲胎蛋白。在本文中，我们研究了各种激素和加压肽对在限定条件下培养的H4细胞血管紧张素原产生的影响。只有糖皮质激素对血管紧张素原的产生有显著影响
