Sadeghiani Marzieh, Bagheri Nader, Shahi Heshmat, Reiisi Somayeh, Rahimian Ghorbanali, Rashidi Reza, Mahsa Majid, Shafigh Mohammedhadi, Salimi Elaheh, Rafieian-Kopaei Mahmoud, Hashemzadeh-Chaleshtori Morteza, Shirzad Hedayatollah
a Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences , Shahrekord , Iran.
b Department of Immunology , School of Public Health, Tehran University of Medical Sciences , Tehran , Iran.
J Immunoassay Immunochem. 2017;38(6):595-607. doi: 10.1080/15321819.2017.1351372. Epub 2017 Jul 12.
Helicobacter pylori (H. pylori) infection has been involved in the pathogenesis of most important gastroduodenal diseases. Matrix metalloproteinases (MMPs) are a large family of zincendopeptidases which play important roles in degradation of extracellular matrix (ECM) and various inflammatory diseases. Therefore, we examined MMP-7 mRNA levels in the gastric mucosa of patients with H. pylori infection and evaluated the effects of virulence factors, such as vacA (vacuolating cytotoxin A) and cagA (cytotoxin-associated gene), in H. pylori-infected patients upon the MMP-7 mRNA mucosal levels. We also determined the correlation between mucosal MMP-7 mRNA levels and the types of disease. Total RNA was extracted from gastric biopsies of 50 H. pylori-infected patients and 50 uninfected individuals. Mucosal MMP-7 mRNA expression level in H. pylori-infected and non-infected gastric biopsies was determined by real-time polymerase chain reaction (PCR). The presences of cagA and vacA virulence factors was evaluated using PCR. MMP-7 expression was significantly higher in biopsies of patients infected with H .pylori compared to uninfected individuals. In addition, mucosal MMP-7 mRNA expression in H. pylori-infected patients significantly associated with the cagA status and the types of disease. Our results suggest that MMP-7 might be involved in the pathogenesis of H. pylori. Peptic ulcer was associated with cag pathogenicity island-dependent MMP-7 upregulation.
幽门螺杆菌（H. pylori）感染与大多数重要的胃十二指肠疾病的发病机制有关。基质金属蛋白酶（MMPs）是一大类锌内肽酶，在细胞外基质（ECM）降解和各种炎症性疾病中起重要作用。因此，我们检测了幽门螺杆菌感染患者胃黏膜中MMP-7 mRNA水平，并评估了幽门螺杆菌感染患者中诸如空泡毒素A（vacA）和细胞毒素相关基因A（cagA）等毒力因子对MMP-7 mRNA黏膜水平的影响。我们还确定了黏膜MMP-7 mRNA水平与疾病类型之间的相关性。从50例幽门螺杆菌感染患者和50例未感染个体的胃活检组织中提取总RNA。通过实时聚合酶链反应（PCR）测定幽门螺杆菌感染和未感染的胃活检组织中黏膜MMP-7 mRNA表达水平。使用PCR评估cagA和vacA毒力因子的存在情况。与未感染个体相比，幽门螺杆菌感染患者活检组织中的MMP-7表达明显更高。此外，幽门螺杆菌感染患者的黏膜MMP-7 mRNA表达与cagA状态和疾病类型显著相关。我们的结果表明，MMP-7可能参与幽门螺杆菌的发病机制。消化性溃疡与cag致病岛依赖性MMP-7上调有关。
