Department of Internal Medicine, College of Medicine, the Catholic University of Korea, Seoul, Korea.
Gastric Cancer. 2010 Aug;13(3):162-9. doi: 10.1007/s10120-010-0552-5. Epub 2010 Sep 5.
The cag pathogenicity island (PAI), a Helicobacter pylori virulence factor, is associated with the pathogenesis of gastric cancer. Matrix metalloproteinase-7(MMP-7) is upregulated in the epithelial cells of gastric cancer. To date, there is limited information available on the role of cag PAI and MMP-7 in precursor lesions. In this study, we aimed to identify virulent H. pylori strains and the expression of MMP-7 in samples of gastric epithelial dysplasia and intramucosal cancer.
One hundred and twelve tissues excised by endoscopic mucosal resection, 76 specimens with gastric epithelial dysplasia and 36 with intramucosal cancer, were examined. All tissue samples were paired with surrounding normal epithelial tissue samples. We performed polymerase chain reaction for cagA and cagL in neoplasia and paired normal specimens, and assessed the matrix metalloproteinase (MMP)-7 expression by immunohistochemical staining.
There was a significant difference in the frequencies of cagA or cagL between specimens with gastric dysplasia and those with intramucosal cancer. We confirmed greater expression of MMP-7 immunoreactivity in intramucosal cancers infected with a virulent H. pylori strain.
Our results suggest that infection with a virulent H. pylori strain was associated with early-stage gastric cancer and that carcinogenesis was associated with cag PAI-dependent MMP-7 upregulation.
cag 致病岛（PAI）是幽门螺杆菌的一种毒力因子，与胃癌的发病机制有关。基质金属蛋白酶-7（MMP-7）在胃癌的上皮细胞中上调。迄今为止，关于 cag PAI 和 MMP-7 在癌前病变中的作用的信息有限。在这项研究中，我们旨在确定有毒幽门螺杆菌菌株和 MMP-7 在胃上皮异型增生和黏膜内癌样本中的表达。
我们检查了 112 个通过内镜黏膜切除术切除的组织，76 个有胃上皮异型增生的标本和 36 个有黏膜内癌的标本。所有组织标本均与周围正常上皮组织标本配对。我们对肿瘤和配对正常标本进行了 cagA 和 cagL 的聚合酶链反应，并通过免疫组织化学染色评估基质金属蛋白酶（MMP）-7 的表达。
在胃异型增生和黏膜内癌标本中，cagA 或 cagL 的频率存在显著差异。我们证实了 MMP-7 免疫反应性在感染有毒幽门螺杆菌菌株的黏膜内癌中表达更高。
我们的结果表明，感染有毒幽门螺杆菌菌株与早期胃癌有关，而癌变与 cag PAI 依赖性 MMP-7 上调有关。
