García-Arrarás J E, Fauquet M, Chanconie M, Smith J
Dev Biol. 1986 Mar;114(1):247-57. doi: 10.1016/0012-1606(86)90400-8.
In the avian embryo, somatostatin-like immunoreactivity (SLI) and adrenergic characteristics appear virtually simultaneously in the developing sympathetic nervous system and adrenal medulla. We have used double-labeling techniques to show that both properties coexist in the same cells. In the quail, not only do all somatostatin-containing cells in the adrenosympathetic system exhibit tyrosine hydroxylase immunoreactivity and possess catecholamines (CA), but this coexistence of the peptidergic and adrenergic phenotypes is already present very early in ontogeny. However, not all adrenergic cells express SLI. The development of sympathoadrenal precursors can be followed in vitro. Adrenergic precursor cells, obtained from the migrating neural crest, differentiate in culture into neuron-like cells that contain SLI and CA. This coexpression can be regulated by the same factors. For instance, corticosterone and progesterone increase SLI content and CA production in the neural crest cell cultures. The ontogeny of the autonomic lineage is discussed in the light of these results.
在禽类胚胎中，生长抑素样免疫反应性（SLI）和肾上腺素能特性几乎同时出现在发育中的交感神经系统和肾上腺髓质中。我们使用双重标记技术表明这两种特性共存于同一细胞中。在鹌鹑中，不仅肾上腺交感神经系统中所有含生长抑素的细胞都表现出酪氨酸羟化酶免疫反应性并含有儿茶酚胺（CA），而且这种肽能和肾上腺素能表型的共存早在个体发育早期就已存在。然而，并非所有肾上腺素能细胞都表达SLI。交感肾上腺前体的发育可以在体外进行追踪。从迁移的神经嵴获得的肾上腺素能前体细胞在培养中分化为含有SLI和CA的神经元样细胞。这种共表达可以由相同的因子调节。例如，皮质酮和孕酮会增加神经嵴细胞培养物中的SLI含量和CA产量。根据这些结果对自主神经谱系的个体发育进行了讨论。
