Maxwell G D, Sietz P D
Dev Biol. 1985 Mar;108(1):203-9. doi: 10.1016/0012-1606(85)90023-5.
The goal of our work is to understand the mechanisms which regulate the differentiation of embryonic neural crest cells into a number of adult cell types, including several classes of neurons. As one aspect of this analysis, the relationship between DNA synthesis and the ontogeny of cells with catecholamines and somatostatin-like immunoreactivity (SLI) in neural crest cell cultures has been investigated. Most of the precursors of the catecholamine- and SLI-positive cells carry out DNA synthesis. As these cells differentiate, their ability to carry out DNA synthesis declines. However, a small percentage of cells continue to synthesize DNA after they become catecholamine or SLI positive. There is no apparent difference between the temporal pattern of DNA synthesis in the precursors of catecholamine-positive cells with SLI and those without SLI. Thus, the time of withdrawal from the cell cycle does not distinguish the lineage of cells that are catecholamine and SLI positive from those that are catecholamine positive and SLI negative.
我们工作的目标是了解调节胚胎神经嵴细胞分化为多种成体细胞类型（包括几类神经元）的机制。作为该分析的一个方面，我们研究了神经嵴细胞培养物中DNA合成与具有儿茶酚胺和生长抑素样免疫反应性（SLI）的细胞个体发育之间的关系。大多数儿茶酚胺和SLI阳性细胞的前体细胞进行DNA合成。随着这些细胞分化，它们进行DNA合成的能力下降。然而，一小部分细胞在成为儿茶酚胺或SLI阳性后仍继续合成DNA。具有SLI的儿茶酚胺阳性细胞前体与不具有SLI的儿茶酚胺阳性细胞前体在DNA合成的时间模式上没有明显差异。因此，退出细胞周期的时间并不能区分儿茶酚胺和SLI阳性细胞系与儿茶酚胺阳性和SLI阴性细胞系。
