Horiguchi Y, Uemura T, Kamata Y, Kozaki S, Sakaguchi G
Infect Immun. 1986 Apr;52(1):31-5. doi: 10.1128/iai.52.1.31-35.1986.
Four hybridoma cell lines producing monoclonal antibodies to Clostridium perfringens enterotoxin were established by fusion of mouse myeloma and spleen cells obtained from mice immunized with the enterotoxin and its toxoid. An enzyme-linked immunosorbent assay indicated that the two antibodies, 2-B-4 and 3-G-10, bound to those regions that were located close each other; the others, 3-B-2 and 2-H-2, bound to other independent regions on the enterotoxin. Release of 51Cr from Vero cells with the enterotoxin was inhibited by either 2-B-4 or 3-G-10, both of which inhibited the binding of 125I-labeled enterotoxin to the cells. Neither binding nor cytotoxicity of the enterotoxin was affected by 2-H-2; 3-B-2 only barely inhibited the binding but neutralized the enterotoxin shown by 51Cr release. It seems justified to conclude that 3-B-2 blocks the toxic action after the enterotoxin has bound to Vero cells.
通过将小鼠骨髓瘤细胞与用产气荚膜梭菌肠毒素及其类毒素免疫的小鼠脾脏细胞进行融合，建立了4株产生抗产气荚膜梭菌肠毒素单克隆抗体的杂交瘤细胞系。酶联免疫吸附测定表明，两种抗体2-B-4和3-G-10与彼此相邻的区域结合；另外两种抗体3-B-2和2-H-2与肠毒素上的其他独立区域结合。2-B-4或3-G-10均可抑制肠毒素诱导的Vero细胞释放51Cr，二者均能抑制125I标记的肠毒素与细胞的结合。2-H-2对肠毒素的结合和细胞毒性均无影响；3-B-2仅轻微抑制结合，但能中和51Cr释放所显示的肠毒素毒性。由此可以合理推断，3-B-2在肠毒素与Vero细胞结合后阻断其毒性作用。
