Marco-Jiménez Francisco, Vicente José Salvador
Institute of Science and Animal Technology, Laboratorio de Biotecnología de la Reproducción, Universidad Politécnica de Valencia, Valencia, Spain.
PLoS One. 2017 Jul 10;12(7):e0180679. doi: 10.1371/journal.pone.0180679. eCollection 2017.
Semen quality has certainly declined over the past few decades, possibly owing to modern lifestyle factors. In this sense, the role of overweight and obesity in the development of subfertility in males has generated a considerable amount of interest in recent years. However, there is no consensus on whether overweight or obesity impaired sperm quality. Thus, based on the ongoing debate about risk factors for subfertility associated with overweight and obesity in men, this study was designed to investigate the effect of overweight on sperm quality parameters and fertility success in randomized controlled trial in a rabbit model. Fourteen male rabbits were randomly assigned to a control group in which nutritional requirements were satisfied or a group fed to satiety from 12 to 32 weeks of age. At 24 weeks of age, semen samples were analysed weekly by conventional semen analysis for 8 weeks. In addition, during the trial female rabbits were artificially inseminated by each male to assess the fertility success and the number of offspring. Young males fed to satiety were associated with a significant increase in body weight (13.6% overweight) and perirenal fat thickness (5%). Male overweight presented a significant decrease in sperm concentration. There were no differences in the remaining sperm parameters. However, male overweight showed a clear and significant decrease in fertility success (control group, 64±8.9% versus fed to satiety group, 35±9.2%), but not in the number of offspring. Taken together, our findings provide new evidence on the loss of fertility induced by overweight in males.
在过去几十年间，精液质量无疑有所下降，这可能归因于现代生活方式因素。从这个意义上讲，超重和肥胖在男性生育力低下发展过程中的作用近年来引发了相当大的关注。然而，对于超重或肥胖是否会损害精子质量，目前尚无定论。因此，基于关于男性超重和肥胖相关生育力低下风险因素的持续争论，本研究旨在通过兔模型的随机对照试验来探究超重对精子质量参数和生育成功率的影响。14只雄性兔子被随机分配到一个满足营养需求的对照组或一个从12周龄到32周龄喂食至饱足的组。在24周龄时，每周通过常规精液分析对精液样本进行8周的分析。此外，在试验期间，用每只雄性兔子对雌性兔子进行人工授精，以评估生育成功率和后代数量。喂食至饱足的年轻雄性兔子体重显著增加（超重13.6%），肾周脂肪厚度增加（5%）。雄性超重导致精子浓度显著降低。其余精子参数没有差异。然而，雄性超重导致生育成功率明显且显著降低（对照组为64±8.9%，喂食至饱足组为35±9.2%），但后代数量没有差异。综上所述，我们的研究结果为超重导致男性生育力丧失提供了新的证据。
