Department of Nanomedicine, Houston Methodist Research Institute , 6670 Bertner Avenue, Houston, Texas 77030, United States.
Department of Cell and Developmental Biology, Weill Medical College of Cornell University , New York, New York 10065, United States.
Anal Chem. 2017 Aug 15;89(16):8273-8281. doi: 10.1021/acs.analchem.7b01288. Epub 2017 Jul 26.
Drug abuse is a major public health problem in many countries in Europe and North America. Currently available platforms for drug abuse assessment are facing technical challenges of nonquantitation, inaccuracy, low throughput, incompatibility with diverse complex specimens, long assay times, and requirement of instrument and/or expertise for readout. Here, we report an integrated competitive volumetric-bar-chart chip (CV-Chip) to assay multiple drug targets at the point-of-care (POC). To the best of our knowledge, it is the first time that a POC platform has been demonstrated to fully address the above-mentioned limitations. We applied this integrated CV-chip platform to assay multiple drugs in 38 patient urine and serum samples and validated the on-chip results with an LC-MS/MS method, indicating a clinical sensitivity and specificity of 0.94 and 1.00, respectively. We further demonstrated that the combination of an on-chip blood separator with the CV-Chip enabled the platform to directly assay finger-prick whole blood samples, which have always been recognized as an ideal biospecimen for POC detections. In summary, this integrated CV-Chip is able to serve as a sensitive, accurate, fast, portable, readout visible, and minimally invasive platform for drug abuse assessment.
药物滥用是欧洲和北美的许多国家面临的主要公共卫生问题。目前用于药物滥用评估的平台面临着非定量、不准确、低通量、与多种复杂样本不兼容、检测时间长以及需要仪器和/或专业知识进行读取等技术挑战。在这里，我们报告了一种集成的竞争性容量-条形图芯片 (CV-Chip)，可在即时护理 (POC) 点检测多种药物靶标。据我们所知，这是首次展示 POC 平台能够完全解决上述限制。我们将这种集成的 CV 芯片平台应用于 38 份患者尿液和血清样本中检测多种药物，并通过 LC-MS/MS 方法验证了芯片上的结果，表明其临床灵敏度和特异性分别为 0.94 和 1.00。我们进一步证明，将芯片上血液分离器与 CV-Chip 相结合，使该平台能够直接检测指血全血样本，这些样本一直被认为是即时检测的理想生物样本。总之，这种集成的 CV-Chip 能够成为一种用于药物滥用评估的灵敏、准确、快速、便携、可读、微创的平台。
