Department of Pathology and Laboratory Medicine, University of Pennsylvania, 3400 Spruce Street, Philadelphia, Pennsylvania 19104, USA.
Analyst. 2019 May 21;144(10):3314-3322. doi: 10.1039/c9an00374f. Epub 2019 Apr 10.
Lateral flow assay (LFA) is a well-established platform for point-of-care (POC) testing due to its low cost and user friendliness. Conventional LFAs provide qualitative or semi-quantitative results and require dedicated instruments for quantitative detection. Here, we developed an "LFA ruler" for quantitative and rapid readout of LFA results, using a 3D printed strip cassette and a simple, inexpensive microfluidic chip. Platinum nanoparticles are used as signal amplification reporters, which catalyze the generation of oxygen to push ink advancement in the microfluidic channel. The concentration of the target is linearly correlated with the ink advancement distance. The entire assay can be completed within 30 minutes without external instruments and complicated operations. We demonstrated quantitative prostate specific antigen testing using the LFA ruler, with a limit of detection of 0.54 ng mL, linear range of 0-12 ng mL, and high correlation with a clinical gold standard assay. The LFA ruler achieves low cost, quantitative, sensitive and rapid detection, which has great potential in POC testing and can be extended to quantify other disease biomarkers.
侧向流分析（LFA）因其成本低、用户友好而成为一种成熟的即时检测（POC）平台。传统的 LFAs 提供定性或半定量结果，并且需要专用仪器进行定量检测。在这里，我们使用 3D 打印条盒和简单、廉价的微流控芯片开发了一种“LFA 标尺”，用于定量和快速读取 LFA 结果。使用铂纳米粒子作为信号放大报告物，其催化氧气的生成以推动微流道中的油墨前进。目标物的浓度与油墨前进距离呈线性相关。整个检测过程可在 30 分钟内完成，无需外部仪器和复杂操作。我们使用 LFA 标尺进行了定量前列腺特异性抗原检测，检测限为 0.54ng/mL，线性范围为 0-12ng/mL，与临床金标准检测具有高度相关性。LFA 标尺实现了低成本、定量、灵敏和快速检测，在 POC 检测中具有很大的潜力，并且可以扩展到定量其他疾病生物标志物。
