Department of Biomedical Science, Centre for Stem Cell Biology, University of Sheffield, Sheffield S10 2TN, UK.
The Broad Institute, Cambridge, MA 02142, USA.
Stem Cell Reports. 2017 Jul 11;9(1):1-4. doi: 10.1016/j.stemcr.2017.05.029.
Pluripotent stem cells may acquire genetic and epigenetic variants during culture following their derivation. At a conference organized by the International Stem Cell Initiative, and held at The Jackson Laboratory, Bar Harbor, Maine, October 2016, participants discussed how the appearance of such variants can be monitored and minimized and, crucially, how their significance for the safety of therapeutic applications of these cells can be assessed. A strong recommendation from the meeting was that an international advisory group should be set up to review the genetic and epigenetic changes observed in human pluripotent stem cell lines and establish a framework for evaluating the risks that they may pose for clinical use.
多能干细胞在其衍生后的培养过程中可能会获得遗传和表观遗传变异。2016 年 10 月，在缅因州巴港的杰克逊实验室举行的国际干细胞倡议组织的会议上，与会者讨论了如何监测和尽量减少此类变异的出现，以及如何评估它们对这些细胞治疗应用安全性的意义。会议强烈建议成立一个国际顾问小组，以审查在人类多能干细胞系中观察到的遗传和表观遗传变化，并建立一个评估它们对临床应用可能构成的风险的框架。
