Horie Masanori, Miura Takamasa, Hirakata Satomi, Hosoyama Akira, Sugino Sakiko, Umeno Aya, Murotomi Kazutoshi, Yoshida Yasukazu, Koike Taisuke
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-Cho, Takamatsu, Kagawa 761-0395, Japan.
Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-49-10 Nishihara, Shibuya-ku, Tokyo 151-0066, Japan.
Exp Anim. 2017 Oct 30;66(4):405-416. doi: 10.1538/expanim.17-0021. Epub 2017 Jul 12.
A relationship between type 2 diabetes mellitus (T2DM) and intestinal flora has been suggested since development of analysis technology for intestinal flora. An animal model of T2DM is important for investigation of T2DM. Although there are some animal models of T2DM, a comparison of the intestinal flora of healthy animals with that of T2DM animals has not yet been reported. The intestinal flora of Tsumura Suzuki Obese Diabetes (TSOD) mice was compared with that of Tsumura, Suzuki, Non Obesity (TSNO) mice in the present study. The TSOD mice showed typical type 2 diabetes symptoms, which were high-fat diet-independent. The TSOD and the TSNO mouse models were derived from the same strain, ddY. In this study, we compared the intestinal flora of TSOD mice with that if TSNO mice at 5 and 12 weeks of age. We determined that that the number of operational taxonomic units (OTUs) was significantly higher in the cecum of TSOD mice than in that of TSNO mice. The intestinal flora of the cecum and that of the feces were similar between the TSNO and the TSOD strains. The dominant bacteria in the cecum and feces were of the phyla Firmicutes and Bacteroidetes. However, the content of some bacterial species varied between the two strains. The percentage of Lactobacillus spp. within the general intestinal flora was higher in TSOD mice than in TSNO mice. In contrast, the percentages of order Bacteroidales and family Lachnospiraceae were higher in TSNO mice than in TSOD mice. Some species were observed only in TSOD mice, such as genera Turicibacter and SMB53 (family Clostridiaceae), the percentage of which were 3.8% and 2.0%, respectively. Although further analysis of the metabolism of the individual bacteria in the intestinal flora is essential, genera Turicibacter and SMB53 may be important for the abnormal metabolism of type 2 diabetes.
自从肠道菌群分析技术发展以来，2型糖尿病（T2DM）与肠道菌群之间的关系就已被提出。T2DM动物模型对于T2DM的研究很重要。虽然有一些T2DM动物模型，但尚未有关于健康动物与T2DM动物肠道菌群比较的报道。在本研究中，对津村铃木肥胖糖尿病（TSOD）小鼠和津村铃木非肥胖（TSNO）小鼠的肠道菌群进行了比较。TSOD小鼠表现出典型的2型糖尿病症状，且与高脂饮食无关。TSOD和TSNO小鼠模型源自同一品系ddY。在本研究中，我们比较了5周龄和12周龄时TSOD小鼠与TSNO小鼠的肠道菌群。我们确定，TSOD小鼠盲肠中的可操作分类单元（OTU）数量显著高于TSNO小鼠。TSNO和TSOD品系之间，盲肠和粪便的肠道菌群相似。盲肠和粪便中的优势菌为厚壁菌门和拟杆菌门。然而，两种品系之间某些细菌种类的含量有所不同。TSOD小鼠肠道菌群中乳酸杆菌属的百分比高于TSNO小鼠。相反，TSNO小鼠中拟杆菌目和毛螺菌科的百分比高于TSOD小鼠。一些菌种仅在TSOD小鼠中被观察到，如Turicibacter属和SMB53（梭菌科），其百分比分别为3.8%和2.0%。虽然对肠道菌群中单个细菌的代谢进行进一步分析至关重要，但Turicibacter属和SMB53可能对2型糖尿病的异常代谢很重要。
