Rached Mouna Ben, Galaverna Gianni, Cirlini Martina, Boujneh Dalenda, Zarrouk Mokhtar, Guerfel Mokhtar
Laboratoire de Biotechnologie de l'Olivier, Centre de Biotechnologie de Borj Cédria.
Department of Food Science, University of Parma, Parco Area delle Scienze.
J Oleo Sci. 2017 Aug 1;66(8):907-915. doi: 10.5650/jos.ess17066. Epub 2017 Jul 12.
The aim of this study examined the characterization of extra virgin olive oil samples from the main cultivar Chemlali, grown in five olive orchards with different soil type (Sandy, Clay, Stony, Brown, Limestone and Gypsum). Volatile compounds were studied using headspace-solid phase micro-extraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) technics. Moreover, the sterol profile was established using gas chromatography-mass spectrometry. 35 different volatile compounds were identified: alcohols, esters, aldehydes, ketones and hydrocarbons. The chemical composition of the volatile fraction was characterized by the preeminence of 2-hexenal (32.75%) and 1-hexanol (31.88%). Three sterols were identified and characterized. For all olive oil samples, ß-sitosterol (302.25 mg/kg) was the most abundant sterol. Interestingly, our results showed significant qualitative and quantitative differences in the levels of the volatile compounds and sterols from oils obtained from olive trees grown in different soil type.
本研究的目的是检测来自主要品种Chemlali的特级初榨橄榄油样品的特性，这些样品产自五个土壤类型不同（沙质、黏土、石质、棕色、石灰岩和石膏）的橄榄园。使用顶空固相微萃取（HS-SPME）和气相色谱-质谱联用（GC-MS）技术研究挥发性化合物。此外，采用气相色谱-质谱联用技术确定甾醇谱。鉴定出35种不同的挥发性化合物：醇类、酯类、醛类、酮类和烃类。挥发性成分的化学组成以2-己烯醛（32.75%）和1-己醇（31.88%）为主。鉴定并表征了三种甾醇。对于所有橄榄油样品，β-谷甾醇（302.25 mg/kg）是含量最丰富的甾醇。有趣的是，我们的结果表明，来自不同土壤类型中生长的橄榄树所产橄榄油的挥发性化合物和甾醇水平在定性和定量上存在显著差异。
