Lee Myung-Hee, Rhee Young-Kyoung, Choi Sang-Yoon, Cho Chang-Won, Hong Hee-Do, Kim Kyung-Tack
Ginseng Research Team, Division of Strategic Food Research, Korea Food Research Institute, Gyeonggi, Republic of Korea.
J Ginseng Res. 2017 Jul;41(3):428-433. doi: 10.1016/j.jgr.2017.03.003. Epub 2017 Mar 19.
In this study, the fermentation of ginseng seeds was hypothesized to produce useful physiologically-active substances, similar to that observed for fermented ginseng root. Ginseng seed was fermented using strains to extract ginseng seed oil, and the extraction yield, color, and quantity of phenolic compounds, fatty acids, and phytosterol were then analyzed.
The ginseng seed was fermented inoculating 1% of each strain on sterilized ginseng seeds and incubating the seeds at 30°C for 24 h. Oil was extracted from the fermented ginseng seeds using compression extraction, solvent extraction, and supercritical fluid extraction.
The color of the fermented ginseng seed oil did not differ greatly according to the fermentation or extraction method. The highest phenolic compound content recovered with the use of supercritical fluid extraction combined with fermentation using the Korea Food Research Institute (KFRI) 1127 strain. The fatty acid composition did not differ greatly according to fermentation strain and extraction method. The phytosterol content of ginseng seed oil fermented with KFRI 1127 and extracted using the supercritical fluid method was highest at 983.58 mg/100 g. Therefore, our results suggested that the ginseng seed oil fermented with KFRI 1127 and extracted using the supercritical fluid method can yield a higher content of bioactive ingredients, such as phenolics, and phytosterols, without impacting the color or fatty acid composition of the product.
在本研究中,假设人参种子发酵会产生有用的生理活性物质,类似于发酵人参根中观察到的情况。使用菌株对人参种子进行发酵以提取人参籽油,然后分析酚类化合物、脂肪酸和植物甾醇的提取率、颜色和含量。
将每种菌株的1%接种到灭菌的人参种子上,在30°C下孵育人参种子24小时,使人参种子发酵。使用压榨提取、溶剂提取和超临界流体提取从发酵的人参种子中提取油。
发酵人参籽油的颜色根据发酵或提取方法的不同差异不大。使用韩国食品研究所(KFRI)1127菌株发酵并结合超临界流体提取回收的酚类化合物含量最高。脂肪酸组成根据发酵菌株和提取方法的不同差异不大。用KFRI 1127发酵并用超临界流体方法提取的人参籽油中植物甾醇含量最高,为983.58毫克/100克。因此,我们的结果表明,用KFRI 1127发酵并用超临界流体方法提取的人参籽油可以产生更高含量的生物活性成分,如酚类和植物甾醇,而不会影响产品颜色或脂肪酸组成。