衰老过程中生长抑素对纯化胰岛B细胞胰岛素分泌的调节能力。

The capability for regulation of insulin secretion by somatostatin in purified pancreatic islet B cells during aging.

作者信息

Magal E, Chaudhuri M, Adelman R C

出版信息

Mech Ageing Dev. 1986 Jan;33(2):139-46. doi: 10.1016/0047-6374(86)90022-9.

Abstract

Pancreatic islet B cells from Sprague-Dawley and Fisher 344 rats aged 3-27 months were separated from A and D cells by centrifugation over a linear percoll density gradient, and incubated in vitro with various concentrations of glucose and somatostatin. Elevation of glucose concentration in the incubation medium from 2.6 to 16.7 mM provokes an insulin secretory response that is independent of rat donor age. Inhibition of the insulin secretory response by somatostatin is independent of rat donor age beyond 12 months. These data indicate that the impaired regulation of insulin secretion during aging observed previously in vivo and in vitro in intact islets may not be intrinsic to the B cells, but instead reflect changes in islet paracrine regulatory mechanisms that relate to the quality and/or quantity of endogenous somatostatin and/or glucagon.

摘要

通过在线性 Percoll 密度梯度上离心，从 3 - 27 月龄的斯普拉格 - 道利大鼠和费希尔 344 大鼠中分离出胰岛 B 细胞，并与 A 细胞和 D 细胞分开，然后在体外与不同浓度的葡萄糖和生长抑素一起孵育。将孵育培养基中的葡萄糖浓度从 2.6 mM 提高到 16.7 mM 会引发胰岛素分泌反应，该反应与大鼠供体年龄无关。生长抑素对胰岛素分泌反应的抑制在 12 个月以上的大鼠供体中与年龄无关。这些数据表明，先前在体内和体外完整胰岛中观察到的衰老过程中胰岛素分泌调节受损可能并非 B 细胞所固有，而是反映了胰岛旁分泌调节机制的变化，这些变化与内源性生长抑素和/或胰高血糖素的质量和/或数量有关。