衰老过程中葡萄糖对胰岛素分泌的调节。

Regulation of insulin secretion by glucose during aging.

作者信息

Sartin J L, Chaudhuri M, Farina S, Adelman R C

出版信息

J Gerontol. 1986 Jan;41(1):30-5. doi: 10.1093/geronj/41.1.30.

DOI:10.1093/geronj/41.1.30

Abstract

The purpose of this study was to investigate the sensitivity of insulin secretion to glucose and its possible relationship to changes in islet glucose utilization during aging. Pancreatic islets of Langerhans were isolated from male Sprague-Dawley rats aged 2- to 24-months and perifused in vitro with glucose. Insulin concentration was determined by radioimmunoassay. Glucose utilization was assessed by measuring the rate of conversion of 3H-5-D-glucose to 3H-H20. The sensitivity of isolated, perifused islets to glucose was reduced during aging. Less glucose metabolism, however, may be required to elicit comparable release of insulin by islets from old than from young rats.

摘要

本研究的目的是调查衰老过程中胰岛素分泌对葡萄糖的敏感性及其与胰岛葡萄糖利用变化的可能关系。从2至24月龄的雄性Sprague-Dawley大鼠分离出胰岛，并在体外进行葡萄糖灌注。通过放射免疫测定法测定胰岛素浓度。通过测量3H-5-D-葡萄糖向3H-H2O的转化速率来评估葡萄糖利用情况。衰老过程中，分离的灌注胰岛对葡萄糖的敏感性降低。然而，与年轻大鼠的胰岛相比，老年大鼠的胰岛可能需要较少的葡萄糖代谢来引发相当的胰岛素释放。