Bronner M E, Cohen A M
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1843-7. doi: 10.1073/pnas.76.4.1843.
Cloned quail melanocytes grown in tissue culture for 8 days or more were injected into 2 1/2-day-old chicken embryos. The pigment cells were placed directly into the somitic lumen by means of an injection micropipette. This technique for introducing marked neural crest cells into host embryos causes far less damage than previous methods which require extirpation and replacement of the neural tube. In addition, small numbers of homogeneous cells can be implanted by this procedure. When injected into one of the posterior somites, cultured pigment cells migrated along the ventral neural crest pathway. Three days after injection the melanocytes had migrated ventral to the dorsal root ganglia and prevertebral and primary sympathetic chain ganglia and were seen associated with the adrenal gland and aortic plexi. Melanocytes were frequently found in or adjacent to the gonads and often had migrated as far as the gut.
将在组织培养中生长8天或更长时间的克隆鹌鹑黑素细胞注射到2.5日龄的鸡胚中。借助注射微量移液器将色素细胞直接置于体节腔中。与以前需要切除和替换神经管的方法相比，这种将标记的神经嵴细胞引入宿主胚胎的技术造成的损伤要小得多。此外，通过该程序可以植入少量均匀的细胞。当注入后体节之一时，培养的色素细胞沿着腹侧神经嵴途径迁移。注射三天后，黑素细胞已迁移到背根神经节、椎前和初级交感神经链神经节的腹侧，并可见与肾上腺和主动脉丛相关。黑素细胞经常在性腺中或性腺附近被发现，并且常常迁移到肠道。
