钝顶螺旋藻可预防高糖诱导的氧化应激介导的心肌成纤维细胞线粒体损伤及凋亡。
Spirulina platensis prevents high glucose-induced oxidative stress mitochondrial damage mediated apoptosis in cardiomyoblasts.
作者信息
Jadaun Pratiksha, Yadav Dhananjay, Bisen Prakash Singh
机构信息
School of Studies in Biotechnology, Jiwaji University, Gwalior, 474011, MP, India.
Department of Medical Biotechnology, Yeungnam University, Gyeongsan, Kangwon-Do, 712-749, Korea.
出版信息
Cytotechnology. 2018 Apr;70(2):523-536. doi: 10.1007/s10616-017-0121-4. Epub 2017 Jul 12.
Abstract
The current study was undertaken to study the effect of Spirulina platensis (Spirulina) extract on enhanced oxidative stress during high glucose induced cell death in H9c2 cells. H9c2 cultured under high glucose (33 mM) conditions resulted in a noteworthy increase in oxidative stress (free radical species) accompanied by loss of mitochondrial membrane potential, release of cytochrome c, increase in caspase activity and pro-apoptotic protein (Bax). Spirulina extract (1 μg/mL), considerably inhibited increased ROS and RNS levels, reduction in cytochrome c release, raise in mitochondrial membrane potential, decreased the over expression of proapoptotic protein Bax and suppressed the Bax/Bcl2 ratio with induced apoptosis without affecting cell viability. Overall results suggest that Spirulina extract plays preventing role against enhanced oxidative stress during high glucose induced apoptosis in cardiomyoblasts as well as related dysfunction in H9c2 cells.
摘要
本研究旨在探讨钝顶螺旋藻提取物对高糖诱导H9c2细胞死亡过程中氧化应激增强的影响。在高糖（33 mM）条件下培养的H9c2细胞，氧化应激（自由基）显著增加，同时伴有线粒体膜电位丧失、细胞色素c释放、半胱天冬酶活性增加和促凋亡蛋白（Bax）增加。钝顶螺旋藻提取物（1μg/mL）可显著抑制活性氧和活性氮水平的升高，减少细胞色素c的释放，提高线粒体膜电位，降低促凋亡蛋白Bax的过表达，并抑制Bax/Bcl2比值，诱导细胞凋亡而不影响细胞活力。总体结果表明，钝顶螺旋藻提取物在心肌成纤维细胞高糖诱导的凋亡过程中，对氧化应激增强及H9c2细胞相关功能障碍具有预防作用。
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