Rinaldi A M, De Leo G, Arzone A, Salcher I, Storace A, Mutolo V
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1916-20. doi: 10.1073/pnas.76.4.1916.
Enucleated halves of sea urchin eggs obtained by centrifugation contain almost all the mitochondrial population of the egg. Removal of the nucleus followed by parthenogenetic activation stimulates the incorporation of [3H]thymidine into the mitochondrial DNA, whereas no such incorportion is observed in activated whole eggs. The block is not the result of a modification in the permeability of the mitochondrial membrane. Electron microscopic observations demonstrated duplication of mitochondrial DNA molecules in activated enucleated halves. No duplication was found in the mitochondrial DNA from activated whole eggs or from nonactivated enucleated halves. We conclude that the cell nucleus exerts a negative control on the activity of the mitochondrial genome through some short-lived nuclear substance(s).
通过离心获得的海胆卵去核半卵包含了卵中几乎所有的线粒体群体。去除细胞核后进行孤雌激活会刺激[3H]胸腺嘧啶核苷掺入线粒体DNA，而在激活的完整卵中未观察到这种掺入。这种阻断不是线粒体膜通透性改变的结果。电子显微镜观察表明，激活的去核半卵中线粒体DNA分子发生了复制。在激活的完整卵或未激活的去核半卵的线粒体DNA中未发现复制现象。我们得出结论，细胞核通过某种短命的核物质对线粒体基因组的活性施加负调控。
