一种测量线粒体质量和活性的方法。
A method for measuring mitochondrial mass and activity.
机构信息
Dipartimento di Biologia Cellulare e dello Sviluppo "A. Monroy", Università degli Studi di Palermo, Viale delle Scienze ed. 16, 90128, Palermo, Italy.
出版信息
Cytotechnology. 2008 Mar;56(3):145-9. doi: 10.1007/s10616-008-9143-2. Epub 2008 Jun 24.
Abstract
Mitochondria, responsible for the energy-generating process essential for the cell metabolism, differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs. Using fluorescent probes specific for mitochondria, Mitotracker Green (MTG) and Orange (MTO), and Confocal Laser-Scanning Microscope (CLSM), we elaborated a method to measure in vivo the mitochondrial mass and activity, in sea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variation of mitochondrial distribution and an increase of activity after fertilization.
摘要
线粒体负责细胞代谢所必需的能量产生过程，其数量、定位和活性因动物细胞和组织的能量需求而有所不同。我们使用特异性标记线粒体的荧光探针 Mitotracker Green（MTG）和 Orange（MTO）以及共聚焦激光扫描显微镜（CLSM），建立了一种在海胆 Paracentrotus lividus 卵和胚胎中测量线粒体质量和活性的体内方法。对捕获图像的分析表明，线粒体的分布发生了变化，受精后活性增加。
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