Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Key Laboratory of Fishery Ecology and Environment, Guangdong Province, South China Sea Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
Sci Total Environ. 2017 Dec 31;607-608:357-366. doi: 10.1016/j.scitotenv.2017.07.040. Epub 2017 Jul 27.
Considerable attention has been paid to the occurrence and abundance of antibiotic resistance genes (ARGs) in aquatic environments. However, the temporal variation and dissemination of ARGs in aquaculture environments and reared organisms need further study. This study investigated the abundance and diversity of ARGs and bacterial community in water source, shrimp pond water, sediment, and shrimps during the rearing period in Pearl River Delta region, South China. The results showed that sul1, qnrD, cmlA, and floR were the predominant ARGs in the aquaculture samples. A trend of decreasing abundance of ARGs was observed for pond water samples during the rearing period, whereas an increasing trend was observed in the sediment and shrimp samples. The total concentration of ARGs in water source was significantly higher than that in shrimp pond water (p<0.05). A significant negative correlation was found between the total concentrations of ARGs in pond waters and sediments (p<0.01). The total abundances of ARGs in intestinal tract of adult shrimps were 4.48-19.0 times higher than those in juvenile shrimps. Similar to water source and pond water, cmlA and sul1 were the predominant ARGs in shrimp intestinal tract. The bacterial community in the shrimp intestinal tract changed greatly from juvenile to adult. The results of the present study indicated that the abundances of ARGs in aquaculture varied temporally during the rearing period. Water source was an important medium disseminating ARGs to the aquaculture environments and reared organisms. Sul1 could be used as a potential indicator for ARGs in both water and sediment in aquaculture in the estuary of the Pearl River Delta, South China. This study represents a case study for the temporal variation of abundance and dissemination of ARGs in aquaculture and is a reference for potential risks to food safety and human health.
人们已经关注到抗生素耐药基因（ARGs）在水生环境中的出现和丰度。然而，水产养殖环境和养殖生物中 ARGs 的时间变化和传播仍需要进一步研究。本研究调查了中国珠江三角洲地区养殖期间水源、虾塘水、沉积物和虾中 ARGs 和细菌群落的丰度和多样性。结果表明，养殖样本中主要的 ARGs 为 sul1、qnrD、cmlA 和 floR。养殖期间，池塘水样中 ARGs 的丰度呈下降趋势，而沉积物和虾样中则呈上升趋势。水源中 ARGs 的总浓度明显高于虾塘水（p<0.05）。池塘水中和沉积物中 ARGs 的总浓度呈显著负相关（p<0.01）。成虾肠道中 ARGs 的总丰度比幼虾高 4.48-19.0 倍。与水源和池塘水相似，cmlA 和 sul1 是虾肠道中主要的 ARGs。虾肠道中的细菌群落从幼虾到成虾发生了很大变化。本研究表明，养殖期间 ARGs 的丰度随时间变化。水源是将 ARGs 传播到水产养殖环境和养殖生物的重要介质。sul1 可作为珠江三角洲河口地区水产养殖水和沉积物中 ARGs 的潜在指示物。本研究代表了水产养殖中 ARGs 丰度和传播的时间变化的案例研究，为食品安全和人类健康的潜在风险提供了参考。
