de Magistris L, Annibale B, Delle Fave G, Salera M, Puoti M, Giordano E, Forte A, Erspamer V
Peptides. 1985;6 Suppl 3:203-8. doi: 10.1016/0196-9781(85)90375-4.
Methanol extracted skins from 84 species of amphibia were screened, measuring by RIAs: gastrin-CCK, VIP, calcitonin, GIP, PP and motilin. G-CCK-like immunoreactivity was found in 97.6%; VIP-like immunoreactivity in 41%; CT-like immunoreactivity in 34%; GIP-like immunoreactivity in 10%; PP-like immunoreactivity in 40% and MT-like immunoreactivity in 60% of the samples. The use of a sequence-specific radioimmunoassay and of gel-chromatography confirmed the caerulein-CCK-8-like nature of the immunoreactive material. Detected amounts of the other peptides (VIP, CT, GIP, PP, MT) were too low for bioassay or chromatographic studies, thus leaving the question open if they are due to some kind of unspecific interferences or, most likely, to species-specificity differences of the used antisera.
对84种两栖动物的甲醇提取物皮肤进行了筛选，采用放射免疫分析法测定：胃泌素-胆囊收缩素（gastrin-CCK）、血管活性肠肽（VIP）、降钙素（CT）、胃抑肽（GIP）、胰多肽（PP）和胃动素（MT）。在97.6%的样本中发现了G-CCK样免疫反应性；41%的样本中有VIP样免疫反应性；34%的样本中有CT样免疫反应性；10%的样本中有GIP样免疫反应性；40%的样本中有PP样免疫反应性；60%的样本中有MT样免疫反应性。使用序列特异性放射免疫分析法和凝胶色谱法证实了免疫反应物质具有雨蛙素-CCK-8样的性质。检测到的其他肽（VIP、CT、GIP、PP、MT）的量过低，无法进行生物测定或色谱研究，因此它们是由于某种非特异性干扰，还是很可能由于所用抗血清的物种特异性差异，这个问题仍然悬而未决。
