Cho Dae-Chul, Ryu Kyoungsu, Kim Kyoung-Tae, Sung Joo-Kyung
Department of Neurosurgery, Kyungpook National University School of Medicine, Daegu, Korea.
Department of Neurosurgery, Halla Hospital, Jeju, Korea.
Korean J Spine. 2017 Jun;14(2):35-40. doi: 10.14245/kjs.2017.14.2.35. Epub 2017 Jun 30.
The purpose of this study was to evaluate the therapeutic effects of combination therapy with curcumin and alendronate on spine fusion surgery in ovariectomized rats.
Thirty-two female Sprague-Dawley rats (12 weeks old) underwent bilateral ovariectomy (OVX). Eight weeks after surgery, animals underwent intertransverse spine fusion at L4-5. The rats were randomly distributed amongst 4 groups; untreated OVX group, curcumin administered group, alendronate administered group, and the combination therapy group. At 8 weeks after fusion surgery, the animals were sacrificed and the fusion mass was assessed by manual palpation, radiographic scan, and micro-computed tomographic scan. In addition, mechanical strength was determined by a 3-point bending test.
Based on the results of manual palpation testing and 3-dimensional micro-computed tomography scanning, solid bone fusion rate was 50% (4 of 8) in the OVX group, 75% (6 of 8) in the alendronate-only and curcumin-only group, and 87.5% (7 of 8) in the combination therapy group, respectively. The combination therapy group had a higher fusion rate compared with the other treatment groups, though not statistically significantly (p>0.05). And the combination therapy group had a significant increase in fusion volume at 8 weeks after spine fusion surgery compared with curcumin-only group (p=0.039). The 3-point bending test showed that combination therapy group had a significantly greater maximal load value compared to that of curcumin-only group (p=0.024).
The present study demonstrated that additional treatment of curcumin and alendronate after spine fusion surgery in rat can promote higher fusion volume, and improve bone mechanical strength.
本研究旨在评估姜黄素与阿仑膦酸钠联合治疗对去卵巢大鼠脊柱融合手术的治疗效果。
32只12周龄雌性Sprague-Dawley大鼠接受双侧卵巢切除术（OVX）。术后8周，动物接受L4-5节段横突间脊柱融合术。大鼠被随机分为4组；未治疗的OVX组、姜黄素给药组、阿仑膦酸钠给药组和联合治疗组。融合手术后8周，处死动物，通过手动触诊、X线扫描和微型计算机断层扫描评估融合块。此外，通过三点弯曲试验测定机械强度。
根据手动触诊测试和三维微型计算机断层扫描结果，OVX组的坚实骨融合率为50%（8只中的4只），仅阿仑膦酸钠组和仅姜黄素组为75%（8只中的6只），联合治疗组为87.5%（8只中的7只）。联合治疗组的融合率高于其他治疗组，但无统计学意义（p>0.05）。与仅姜黄素组相比，联合治疗组在脊柱融合手术后8周的融合体积显著增加（p=0.039）。三点弯曲试验表明，联合治疗组的最大负荷值显著高于仅姜黄素组（p=0.024）。
本研究表明，大鼠脊柱融合手术后额外给予姜黄素和阿仑膦酸钠可促进更高的融合体积，并提高骨机械强度。
