中国北京婴儿肉毒中毒的分子与流行病学特征
Molecular and Epidemiological Characterization of Infant Botulism in Beijing, China.
作者信息
Dong Yin Ping, Wang Wei, Jiang Tao, Xu Jin, Han Chun Hui, Yan Shao Fei, Fanning Séamus, Li Ying, Ma Xiao Chen, Zhang Di, Zhao Yao, Zeng Biao, Li Feng Qin
机构信息
Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment (CFSA), Beijing 100021, China.
Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment (CFSA), Beijing 100021, China; School of Public Health, Physiotherapy & Sports Science, University College Dublin, Dublin D04 N2E5, Ireland.
出版信息
Biomed Environ Sci. 2017 Jun;30(6):460-464. doi: 10.3967/bes2017.061.
Laboratory-based pathogen isolation, identification, and toxicity determination were performed on samples from a suspected case of infant botulism. Mice injected with cultures generated from the enema sample and ingested Powered infant formula (PIF) presented typical signs of botulism. Antitoxins to polyvalent botulinum neurotoxins (BoNTs) and monovalent BoNT type B antitoxin had protective effects. Clostridium botulinum isolated from the enema and residual PIF samples were positive for type B toxin. Pulsed-field gel electrophoresis (PFGE) revealed that the two strains of C. botulinum isolated from the two samples produced indistinguishable pulsotypes. These findings confirmed this case of type B infant botulism associated with the ingestion of PIF contaminated by type B C. botulinum spores.
对一例疑似婴儿肉毒中毒病例的样本进行了基于实验室的病原体分离、鉴定和毒性测定。注射了灌肠样本培养物并摄入婴儿配方奶粉（PIF）的小鼠出现了典型的肉毒中毒症状。多价肉毒杆菌神经毒素（BoNTs）抗毒素和单价B型肉毒杆菌抗毒素具有保护作用。从灌肠液和剩余PIF样本中分离出的肉毒梭菌B型毒素呈阳性。脉冲场凝胶电泳（PFGE）显示，从两个样本中分离出的两株肉毒梭菌产生了难以区分的脉冲型。这些发现证实了这例与摄入被B型肉毒梭菌孢子污染的PIF相关的B型婴儿肉毒中毒病例。
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